在厌氧条件下，减少蛋白质的氧化，并加速水溶性双（三唑）配体的铜催化“点击”反应。
Anaerobic conditions to reduce oxidation of proteins and to accelerate the copper-catalyzed "Click" reaction with a water-soluble bis(triazole) ligand.
机构信息
Department of Chemistry, University of Houston, Houston, TX 77204, USA.
出版信息
Chem Commun (Camb). 2011 Mar 21;47(11):3186-8. doi: 10.1039/c0cc05376g. Epub 2011 Jan 31.
Abstract
Oxidation of protein (bovine albumin serum) by air still occurred under the copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction conditions even in the presence of a Cu(I)-stabilizing tris(triazole) ligand. Anaerobic conditions not only avoided the oxidation of the protein, but also greatly accelerated the CuAAC reaction using a water-soluble bis(triazole) Cu(I) ligand.
摘要
即使在铜（I）稳定的三唑配体存在下，铜催化的叠氮化物-炔烃环加成（CuAAC）反应条件下，蛋白质（牛血清白蛋白）仍会被空气氧化。在无氧条件下，不仅可以避免蛋白质的氧化，而且还可以使用水溶性双（三唑）铜（I）配体极大地加速 CuAAC 反应。
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