Luo Ting Rong, Liao Su-Huan, Wu Xian-Shi, Feng Li, Yuan Zhao-Xia, Li Hui, Liang Jing-Jing, Meng Xian-Ming, Zhang Hong-Yun
College of Animal Sciences and Veterinary Medicine, Guangxi University, Nanning, China.
Virus Genes. 2011 Jun;42(3):347-54. doi: 10.1007/s11262-011-0578-8. Epub 2011 Feb 2.
In this study, suspected classical swine fever (CSF) samples from the Guangxi Province of China were obtained from pigs with acute CSF, aborted fetuses, newborn pigs that died at 1-2 days of age, tonsils of healthy pigs, and leukocytes of immunized sows during 2001-2009. About 92 of 775 samples were found to be positive by RT-PCR, and 41 isolates were obtained. Phylogenetic analysis was performed on the 31 isolates by sequencing the E2 gene, and the isolates were found to cluster into two groups: (1) isolates from aborted fetuses (except GXGZ02), deceased newborn baby pigs, tonsils of healthy pigs, and leukocytes of immunized sows belonged to group 1.1, along with vaccine strain, HCLV, and standard virulent strain, Shimen, of China, and (2) 20 isolates from pigs with acute CSF belonged to group 2.1, 13 of which were clustered into subgroup 2.1b with isolates from other provinces of China, and 7 of which were clustered into subgroup 2.1a with isolates from Italy and Germany.
在本研究中，2001年至2009年期间，从中国广西省患有急性猪瘟的猪、流产胎儿、1至2日龄死亡的新生仔猪、健康猪的扁桃体以及免疫母猪的白细胞中获取疑似经典猪瘟（CSF）样本。775份样本中约92份经逆转录聚合酶链反应（RT-PCR）检测呈阳性，并获得了41株分离株。通过对31株分离株的E2基因进行测序进行系统发育分析，发现这些分离株分为两组：（1）来自流产胎儿（GXGZ02除外）、死亡新生仔猪、健康猪扁桃体以及免疫母猪白细胞的分离株属于1.1组，与中国的疫苗株HCLV和标准强毒株石门株在一起；（2）来自患有急性猪瘟的猪的20株分离株属于2.1组，其中13株与来自中国其他省份的分离株聚集在2.1b亚组，7株与来自意大利和德国的分离株聚集在2.1a亚组。
