J Dermatol Sci. 2011 Mar;61(3):211-5. doi: 10.1016/j.jdermsci.2010.12.009. Epub 2011 Jan 13.
L-Arginine (L-Arg) is a conditionally essential amino acid for humans, which is the substrate for both arginase (ARG) and the inducible form of nitric oxide synthase (iNOS) enzymes. Whether L-Arg metabolism has detrimental or beneficial influence on the tumor growth depends on local up regulation of the NOS or ARG pathways at the tumor site.
To evaluate the expression profile of ARG and iNOS in various histological subtypes of soft tissue sarcomas (STSs).
A series of 81 adult STSs were tested for ARG1, ARG2 and iNOS expression by immunohistochemical analysis.
ARG1, ARG2 and iNOS expression was found in tumor cells of all cases of STSs except dermatofibrosarcoma protuberans (DFSP) in a cytoplasmic pattern. However, there was no significant correlation found between ARG, iNOS expression and histopathological parameters. Conversely, the majority of DFSP were devoid of ARG and iNOS expression, while only two cases showed focal and weak expression.
Overexpression of L-Arg-metabolizing enzymes ARG and iNOS in tumor cells of all of the STS cases except DFSP may have a role in mediating the biological processes which characterize STSs. New knowledge of the regulation of arginine metabolism in tumor tissues is key to designing sound therapeutic means to effectively prevent tumorigenesis. Further studies are needed to clarify the absence of ARG and iNOS staining in DFSP.
精氨酸（L-Arg）是人体条件必需氨基酸，是精氨酸酶（ARG）和诱导型一氧化氮合酶（iNOS）两种酶的底物。L-Arg 代谢对肿瘤生长是有害还是有益，取决于肿瘤部位 NOS 或 ARG 途径的局部上调。
评估 ARG 和 iNOS 在各种组织学亚型软组织肉瘤（STSs）中的表达谱。
通过免疫组织化学分析检测 81 例成人 STSs 中 ARG1、ARG2 和 iNOS 的表达情况。
除隆突性皮肤纤维肉瘤（DFSP）外，所有 STSs 的肿瘤细胞均呈细胞质模式表达 ARG1、ARG2 和 iNOS。然而，ARG、iNOS 表达与组织病理学参数之间无显著相关性。相反，大多数 DFSP 缺乏 ARG 和 iNOS 表达，只有 2 例表现为局灶性和弱阳性表达。
除 DFSP 外，所有 STSs 肿瘤细胞中 L-Arg 代谢酶 ARG 和 iNOS 的过度表达可能在介导 STSs 的生物学过程中起作用。了解肿瘤组织中精氨酸代谢的调节对于设计有效的治疗方法以有效预防肿瘤发生至关重要。需要进一步研究来阐明 DFSP 中 ARG 和 iNOS 染色缺失的原因。
