State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University , Nanjing 210096, P.R. China.
Biomacromolecules. 2011 Mar 14;12(3):551-9. doi: 10.1021/bm100886w. Epub 2011 Feb 4.
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable polyester, has been a good candidate of biomaterial employed in tissue engineering. However, the PHBV film is hydrophobic and has no recognition sites for cell attachment. In this study, PHBV films are activated by ammonia plasma treatment to produce amino groups on the surface, followed by sequential reactions with a heterobifunctional cross-linker containing a segment of poly(ethylene glycol) (PEG) and further with RGD-containing peptides. XPS analyses of modified surfaces after each reaction step reveal that the RGD-containing peptides have been covalently grafted onto PHBV films. The result of cell viability assay indicates that the RGD-modified PHBV films exhibit a distinctly improved cellular compatibility. Moreover, according to the results of serum adsorption tests by optical waveguide lightmode spectroscopy (OWLS) and fibrinogen adsorption tests by enzyme-linked immunosorbent assay (ELISA) on unmodified and modified PHBV surfaces, the introduced PEG chains can significantly decrease the nonspecific adsorption of proteins from serum and fibrinogen from plasma, thus decreasing the risk of thrombus formation and improving the blood compatibility of implanted materials.
聚（3-羟基丁酸酯-co-3-羟基戊酸酯）（PHBV）是一种可生物降解的聚酯，已成为组织工程中生物材料的良好候选材料。然而，PHBV 薄膜是疏水性的，没有细胞附着的识别位点。在这项研究中，通过氨等离子体处理使 PHBV 薄膜表面活化，生成氨基，然后依次与含有聚乙二醇（PEG）片段的杂双功能交联剂和含有 RGD 的肽反应。对每个反应步骤后改性表面的 XPS 分析表明，含有 RGD 的肽已被接枝到 PHBV 薄膜上。细胞活力测定的结果表明，RGD 修饰的 PHBV 薄膜表现出明显改善的细胞相容性。此外，根据未修饰和修饰的 PHBV 表面的光波导光模式光谱（OWLS）的血清吸附试验和酶联免疫吸附试验（ELISA）的纤维蛋白原吸附试验的结果，引入的 PEG 链可以显著降低来自血清的蛋白质和来自血浆的纤维蛋白原的非特异性吸附，从而降低血栓形成的风险，提高植入材料的血液相容性。
