Exact Sciences Department, Banat's University of Agricultural Sciences and Veterinary Medicine from Timisoara, Calea Aradului no.119, 300645 Timisoara, Romania.
J Nanobiotechnology. 2011 Feb 4;9:3. doi: 10.1186/1477-3155-9-3.
The use of natural products based on aqueous extract of propolis and lycopene in the skin's protective mechanisms against UVA radiation was evaluated by means of experimental acute inflammation on rat paw edema. The aim of the present study was to evaluate the harmlessness of propolis - lycopene system through evaluation of skin level changes and anti-inflammatory action. The regenerative and protective effect of the aqueous propolis and lycopene extract is based on its richness in biologically active substances such as: tocopherols, flavonoids, amino acids, polyunsaturated fatty acids, the chlorophyll pigment, all substances with strong antioxidant activity, that modify the oxidative stress, mainly by reducing the prooxidant processes and enhancing the antioxidant ones. These substances participate in the synthesis of prostaglandins and phospholipids components of cell membrane thus enhancing skin protection mechanisms.
The experimental systems offered a sustained release of the drug, in vitro, for aim eight hours. The prepared formulations aim did not reveal a deteriorating effect on tissues. They proved a better therapeutic efficiency Compared to standard suspension, they provided a better therapeutic efficiency coupled with extended time interval of tested parameters (24 hours). Preliminary examination of tissues showed that the experimental formulations did not irritate. Local application of propolis and lycopene aqueous extract nanoemulsion has a high potential both regarding its efficiency (the analgesic effect) and therapeutic safety.
This study demonstrates that propolis and lycopene extract nanoemulsions, preparations contains active substances, can confer better therapeutic effects than those of the conventional formulations, based on local control-release of dozed form, for a longer period of time, which probably improve its efficiency and skin acceptance, meaning a better compliance. The information obtained in the present study suggests that administration of propolis and lycopene aqueous extract nanoemulsion is safe. The preparation can be useful for further preclinical studies lycopene embedded in aqueous propolis extract to be used in pharmaceuticals (targeted medical therapy).
通过评估大鼠爪肿胀的急性炎症实验，评估了基于蜂胶和番茄红素水提取物的天然产物在皮肤对抗 UVA 辐射的保护机制中的作用。本研究旨在通过评估皮肤水平变化和抗炎作用来评估蜂胶-番茄红素系统的无害性。蜂胶和番茄红素水提取物的再生和保护作用基于其丰富的生物活性物质，如生育酚、类黄酮、氨基酸、多不饱和脂肪酸、叶绿素色素，所有这些物质都具有很强的抗氧化活性，可改变氧化应激，主要通过减少促氧化剂过程和增强抗氧化剂过程。这些物质参与前列腺素和细胞膜磷脂的合成，从而增强皮肤保护机制。
实验系统提供了持续释放药物的效果，体外持续 8 小时。所制备的配方对组织没有显示出恶化作用。与标准混悬剂相比，它们提供了更好的治疗效果，同时延长了测试参数的时间间隔（24 小时）。对组织的初步检查表明，实验配方没有刺激性。蜂胶和番茄红素水提取物纳米乳液的局部应用具有很高的潜力，无论是在效率（镇痛效果）还是治疗安全性方面。
本研究表明，基于局部控制释放剂量形式，在更长的时间内，含有活性物质的蜂胶和番茄红素提取物纳米乳液制剂比常规制剂具有更好的治疗效果，这可能会提高其效率和皮肤接受度，从而提高患者的顺应性。本研究获得的信息表明，蜂胶和番茄红素水提取物纳米乳液的给药是安全的。该制剂可用于进一步的临床前研究，将番茄红素嵌入水基蜂胶提取物中，用于制药（靶向药物治疗）。
