比较 VersaTREK 血培养系统与 Bactec9240 系统在疑似血流感染患者中的应用。
Comparison of the VersaTREK blood culture system against the Bactec9240 system in patients with suspected bloodstream infections.
机构信息
Department of Medical Microbiology, University of Pretoria and Tshwane Academic Division of the National Health Laboratory Service (NHLS), Pretoria, South Africa.
出版信息
Ann Clin Microbiol Antimicrob. 2011 Feb 5;10:4. doi: 10.1186/1476-0711-10-4.
BACKGROUND
To evaluate the VersaTREK (TREK Diagnostic Systems, Cleveland, Ohio) blood culture system against the Bactec9240 (BD Microbiology, Cockeysville, MD), for the recovery of bloodstream pathogens.
METHODS
Venous blood from patients with suspected bacterial sepsis was evenly distributed into bottles of each system. Positive signals were recorded and bottles processed onto standard media for organism recovery. False positive signals were regarded if no organisms were seen on Gram stain and no growth was observed.
RESULTS
177 bottles were available for analysis; the Bactec9240 system yielded 43 positive, 134 negative results and no false positive signals. The VersaTREK system had 58 positive signals with 14 being false positives.
CONCLUSIONS
In our setting with high background burden of immuno-compromised patients, the VersaTREK system compared favourably with the Bactec9240 in recovering blood stream aerobic and facultative anaerobic pathogens from patients with suspected bacterial sepsis. A concern is the high false positivity rate. Due to its versatility to accommodate small and large workloads as well as using smaller volumes of blood, this system may establish itself as a useful alternative for the recovery of bloodstream pathogens.
背景
评估 VersaTREK（TREK 诊断系统，俄亥俄州克利夫兰）与 Bactec9240（BD 微生物学，马里兰州考克斯堡）血培养系统对血流感染病原体的检测能力。
方法
将疑似菌血症患者的静脉血均匀分配到各系统的培养瓶中。记录阳性信号，并将培养瓶按标准方法处理以进行病原体回收。如果革兰氏染色未见病原体且无生长，则视为假阳性信号。
结果
共分析了 177 个培养瓶；Bactec9240 系统得到 43 个阳性、134 个阴性和 0 个假阳性结果。VersaTREK 系统有 58 个阳性信号，其中 14 个为假阳性。
结论
在我们这种免疫功能低下患者背景负担较高的环境中，VersaTREK 系统与 Bactec9240 系统相比，从疑似细菌性败血症患者中检测到血流需氧和兼性厌氧病原体的能力较好。一个关注点是较高的假阳性率。由于其多功能性，可适应小和大工作量以及使用较小体积的血液，该系统可能成为检测血流感染病原体的有用替代方法。
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