National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Nat Struct Mol Biol. 2011 Mar;18(3):309-15. doi: 10.1038/nsmb.2008. Epub 2011 Feb 6.
CP29, one of the minor light-harvesting complexes of higher-plant photosystem II, absorbs and transfers solar energy for photosynthesis and also has important roles in photoprotection. We have solved the crystal structure of spinach CP29 at 2.80-Å resolution. Each CP29 monomer contains 13 chlorophyll and 3 carotenoid molecules, which differs considerably from the major light-harvesting complex LHCII and the previously proposed CP29 model. The 13 chlorophyll-binding sites are assigned as eight chlorophyll a sites, four chlorophyll b and one putative mixed site occupied by both chlorophylls a and b. Based on the present X-ray structure, an integrated pigment network in CP29 is constructed. Two special clusters of pigment molecules, namely a615-a611-a612-Lut and Vio(Zea)-a603-a609, have been identified and might function as potential energy-quenching centers and as the exit or entrance in energy-transfer pathways.
CP29 是高等植物光系统 II 中的一个次要捕光复合物，它吸收和传递太阳能用于光合作用，并且在光保护中也具有重要作用。我们已经解决了菠菜 CP29 的晶体结构，分辨率为 2.80 Å。每个 CP29 单体包含 13 个叶绿素和 3 个类胡萝卜素分子，这与主要的捕光复合物 LHCII 和以前提出的 CP29 模型有很大的不同。13 个叶绿素结合位点被分配为 8 个叶绿素 a 位点、4 个叶绿素 b 和一个由叶绿素 a 和 b 共同占据的假定混合位点。基于目前的 X 射线结构，构建了 CP29 中的一个综合色素网络。已经鉴定出两个特殊的色素分子簇，即 a615-a611-a612-Lut 和 Vio（玉米）-a603-a609，它们可能作为潜在的能量猝灭中心以及能量转移途径的出口或入口发挥作用。
