Department of Biological Sciences, University of North Texas, 1704 West Mulberry, Room 215, Denton, TX 76208, USA.
Bull Environ Contam Toxicol. 2011 Mar;86(3):247-51. doi: 10.1007/s00128-011-0200-8. Epub 2011 Feb 8.
The presence of pharmaceuticals in the environment has become an important topic of discussion with respect to pharmaceutical absorption, metabolism and elimination in fish. This study investigates the metabolism of ibuprofen by rainbow trout (Oncorhynchus mykiss). In vitro metabolic loss of parent compound was measured in gill and liver S9 and microsomal fractions. Metabolite analysis found 2-hydroxy-ibuprofen as the major metabolite in uninduced S9 fractions. Supplementing S9 fractions with UDPGA did not significantly enhance metabolism. Additionally, assays involving the induction and inhibition of specific CYP isozymes support CYP1A2 as a possible metabolic pathway in fish.
环境中药物的存在已成为一个重要的讨论话题，涉及鱼类对药物的吸收、代谢和消除。本研究调查了布洛芬在虹鳟（Oncorhynchus mykiss）中的代谢情况。在鳃和肝 S9 和微粒体部分中测量了母体化合物的体外代谢损失。代谢产物分析发现 2-羟基布洛芬是未诱导 S9 部分中的主要代谢产物。用 UDPGA 补充 S9 部分并没有显著增强代谢。此外，涉及特定 CYP 同工酶诱导和抑制的测定支持 CYP1A2 是鱼类中可能的代谢途径。
