Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
J Appl Oral Sci. 2010 Dec;18(6):621-4. doi: 10.1590/s1678-77572010000600014.
The aim of this study was to investigate through scanning electron microscopy (SEM) the cleaning of root canal walls after the use of experimental propolis or calcium hydroxide root canal dressings.
Twenty single-rooted teeth were used. After conventional cleaning and shaping procedures and removal of the smear layer with 17% EDTA, the teeth were divided into four groups according to the medication used (N=5): Group I (control) - No drug, Group II - Calcium hydroxide dressing, Group III - Propolis paste A70D and Group IV - Propolis paste D70D. The medications were introduced into the root canals and maintained for 7 days, then removed with a K-file and 5 mL of 1% sodium hypochlorite irrigation. Finally, the canals were flushed with 2 mL of 17% EDTA for 3 min. For SEM analysis, the roots were cleaved and microphotographs from the middle third of the root canal were taken at 750x. The cleaning of the root canal walls was determined by the number of open dentinal tubules as verified with the software Image Tool 3.1. The statistical analysis was performed by ANOVA and Tukey's test (p<0.05).
The results showed no statistically significant difference between the calcium hydroxide and propolis groups.
The experimental propolis pastes presented acceptable physical characteristics to be used as intracanal medicaments.
本研究旨在通过扫描电子显微镜(SEM)观察使用实验蜂胶或氢氧化钙根管封药后根管壁的清洁情况。
使用 20 颗单根牙。在进行常规的清洁和成形程序以及用 17% EDTA 去除玷污层后,根据使用的药物将牙齿分为四组(N=5):I 组(对照组)-无药物,II 组-氢氧化钙封药,III 组-蜂胶糊 A70D,IV 组-蜂胶糊 D70D。将药物引入根管并保持 7 天,然后用 K 锉和 5 毫升 1%次氯酸钠冲洗去除。最后,用 2 毫升 17% EDTA 冲洗 3 分钟。对于 SEM 分析,将根劈开,并在根管中部的第三个位置以 750x 拍摄微照片。用 Image Tool 3.1 软件验证,通过开放牙本质小管的数量来确定根管壁的清洁程度。采用方差分析和 Tukey 检验进行统计学分析(p<0.05)。
结果显示,氢氧化钙组和蜂胶组之间无统计学差异。
实验蜂胶糊具有可接受的物理特性,可作为根管内药物使用。