胡桃叶和枝中的抗增殖三萜类化合物对 HSC-T6 细胞的作用。

Antiproliferative triterpenes from the leaves and twigs of Juglans sinensis on HSC-T6 cells.

机构信息

College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Daehak-Dong, Gwanak-Gu, Seoul 151-742, Republic of Korea.

出版信息

J Nat Prod. 2011 Apr 25;74(4):751-6. doi: 10.1021/np1008202. Epub 2011 Feb 10.

DOI:10.1021/np1008202

PMID:21309591

Abstract

Bioassay-guided fractionation of an 80% MeOH extract of leaves and twigs of Juglan sinensis has resulted in the isolation of four new triterpenes (1-4) and 17 known triterpenes (5-21). The new compounds were determined to be 1-oxo-3β,23-dihydroxyolean-12-en-28-oic acid 28-O-β-D-glucopyranoside (1), 1-oxo-3β-hydroxyolean-18-ene (2), 3β,23-dihydroxyurs-12-en-28-oic acid 28-O-β-D-glucopyranoside (3), and 3β,22α-dihydroxyurs-12-en-28-oic acid 28-O-β-D-glucopyranoside (4) by spectroscopic analysis. Compounds 2, 13, 15, and 21 showed antiproliferative activities (14.2, 14.8, 15.6, and 11.0% at 100 μM, respectively) in HSC-T6 cells. Flow cytometry assays revealed that these compounds inhibited HSC-T6 proliferation by inducing apoptosis.

摘要

采用生物活性导向方法，对胡桃科核桃属植物青钱柳叶和枝的 80%甲醇提取物进行分离，得到了 4 个新三萜（1-4）和 17 个已知三萜（5-21）。通过光谱分析，确定这些新化合物分别为 1-氧代-3β,23-二羟基齐墩果-12-烯-28-酸 28-O-β-D-吡喃葡萄糖苷（1）、1-氧代-3β-羟基齐墩果-18-烯（2）、3β,23-二羟基熊果酸-12-烯-28-酸 28-O-β-D-吡喃葡萄糖苷（3）和 3β,22α-二羟基熊果酸-12-烯-28-酸 28-O-β-D-吡喃葡萄糖苷（4）。化合物 2、13、15 和 21 在 HSC-T6 细胞中表现出抗增殖活性（在 100μM 时分别为 14.2%、14.8%、15.6%和 11.0%）。流式细胞术分析表明，这些化合物通过诱导细胞凋亡抑制 HSC-T6 增殖。