College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Daehak-Dong, Gwanak-Gu, Seoul 151-742, Republic of Korea.
Bioorg Med Chem Lett. 2012 Mar 1;22(5):2079-83. doi: 10.1016/j.bmcl.2012.01.010. Epub 2012 Jan 13.
Bioassay-guided fractionation of an 80% MeOH extract of Juglan sinensis leaves and twigs has resulted in the isolation of three new triterpenes (1-3) and two new sesquiterpenes (4-5) along with two known sesquiterpenes (6-7). The new compounds were determined to be 3β, 11α, 19α, 24, 30-pentahydroxy-20β, 28-epoxy-28β-methoxy-ursane (1), 1α, 3β-dihydroxy-olean-18-ene (2), 2α, 3α, 23-trihydroxy-urs-12-en-28-oic acid 28-O-β-d-glucopyranoside (3), (4S, 5S, 7R, 8R, 14R)-8, 11-dihydroxy-2, 4-cyclo-eudesmane (4), 15-hydroxy-α-eudesmol-11-O-β-d-glucopyranoside (5), by spectroscopic analysis. The cytotoxicity of compounds (1-7) against four cancer cell lines such as B16F10, Hep-2, MCF-7 and U87-MG was evaluated. Compounds 1, 2, 6 and 7 showed potent cytotoxicity against all of four cancer cell lines, respectively.
从胡桃科植物青钱柳叶和嫩枝的 80%甲醇提取物中进行生物活性导向分离，得到了三个新的三萜（1-3）和两个新的倍半萜（4-5），以及两个已知的倍半萜（6-7）。新化合物被确定为 3β, 11α, 19α, 24, 30-五羟基-20β, 28-环氧-28β-甲氧基-乌苏烷（1）、1α, 3β-二羟基-齐墩果-18-烯（2）、2α, 3α, 23-三羟基-熊-12-烯-28-酸 28-O-β-D-吡喃葡萄糖苷（3）、(4S, 5S, 7R, 8R, 14R)-8, 11-二羟基-2, 4-环-桉烷（4）、15-羟基-α-桉烷-11-O-β-D-吡喃葡萄糖苷（5），通过光谱分析。测定了化合物（1-7）对 B16F10、Hep-2、MCF-7 和 U87-MG 四种癌细胞系的细胞毒性。化合物 1、2、6 和 7 对所有四种癌细胞系均表现出较强的细胞毒性。
