Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Buenos Aires, Argentina; National Research Council (CONICET), Argentina.
Physiol Behav. 2011 Jun 1;103(3-4):315-20. doi: 10.1016/j.physbeh.2011.01.026. Epub 2011 Feb 18.
Circadian rhythms govern a wide variety of physiological and metabolic functions in most organisms through neural networks, hormones and gene expression. In this work, we studied the circadian variation in metabolic variables of adult C. elegans such as food consumption, pharyngeal contractions, defecation and oxygen consumption. Feeding behavior was clearly rhythmic under LD conditions, with a non-significant trend under DD conditions. In addition, a daily and circadian variation in muscle contraction of the pharynx was observed. Oxygen consumption also showed a circadian fluctuation with a maximum in the middle of the night (a peak was found around ZT18/CT18). Furthermore, defecation behavior also showed a daily variation in the N2 strain (wild type). This work demonstrates that in the adult nematode C. elegans metabolic variables vary daily. In summary, our results will allow us to take full advantage of this widely used animal model (including research in genetics, ageing and developmental biology) for studies in Chronobiology.
昼夜节律通过神经网络、激素和基因表达来控制大多数生物体的广泛生理和代谢功能。在这项工作中，我们研究了成年秀丽隐杆线虫的代谢变量的昼夜变化，如食物消耗、咽收缩、排便和耗氧量。在 LD 条件下，摄食行为明显呈节律性，在 DD 条件下呈非显著性趋势。此外，还观察到咽肌收缩的日常和昼夜变化。耗氧量也表现出昼夜波动，夜间中期达到最大值（大约在 ZT18/CT18 时出现峰值）。此外，N2 品系（野生型）的排便行为也呈现出每日变化。这项工作表明，在成年秀丽隐杆线虫中，代谢变量每天都在变化。总之，我们的结果将使我们能够充分利用这种广泛使用的动物模型（包括遗传学、衰老和发育生物学研究）来进行时间生物学研究。
