Bullman Susan, Corcoran Daniel, O'Leary James, Lucey Brigid, Byrne Deirdre, Sleator Roy D
Department of Biological Sciences, Cork Institute of Technology, Cork, Ireland.
FEMS Immunol Med Microbiol. 2011 Mar;61(2):228-30. doi: 10.1111/j.1574-695X.2010.00760.x. Epub 2010 Dec 6.
A total of 7194 faecal samples collected over a 1-year period from patients presenting with diarrhoea were screened for Campylobacter spp. using EntericBio(®) , a multiplex-PCR system. Of 349 Campylobacter-positive samples, 23.8% were shown to be Campylobacter ureolyticus, using a combination of 16S rRNA gene analysis and highly specific primers targeting the HSP60 gene of this organism. This is, to the best of our knowledge, the first report of C. ureolyticus in the faeces of patients presenting with gastroenteritis and may suggest a role for this organism as an emerging enteric pathogen.
在为期1年的时间里,共收集了7194份腹泻患者的粪便样本,使用多重聚合酶链反应(PCR)系统EntericBio(®) 对弯曲杆菌属进行筛查。在349份弯曲杆菌阳性样本中,通过16S rRNA基因分析和针对该菌HSP60基因的高特异性引物相结合的方法,有23.8%被鉴定为解脲弯曲杆菌。据我们所知,这是首次关于解脲弯曲杆菌在肠胃炎患者粪便中的报道,这可能表明该菌作为一种新兴肠道病原体发挥了作用。