Sutor D J, Percival J M, Doonan S
Clin Chim Acta. 1978 Oct 16;89(2):273-8. doi: 10.1016/0009-8981(78)90325-x.
Normal urine has been examined for substances which inhibit formation of calcium phosphate. A separation scheme involving ultrafiltration, precipitation, electrophoresis and paper chromatography was devised to isolate these substances. Contrary to what has been suggested in the literature for many years, the urines examined did not contain a potent unidentified inhibitor. The major anionic inhibitors were citric acid, pyrophosphate and isocitric acid. These substances together with a small contribution from the cations appeared to account for most, if not all, of the inhibitory activity of urine.
已对正常尿液中的抑制磷酸钙形成的物质进行了检测。设计了一种包括超滤、沉淀、电泳和纸色谱法的分离方案来分离这些物质。与文献中多年来所暗示的情况相反，所检测的尿液中并不含有一种强效的未鉴定抑制剂。主要的阴离子抑制剂是柠檬酸、焦磷酸和异柠檬酸。这些物质连同阳离子的少量贡献似乎占了尿液中大部分（如果不是全部）抑制活性的原因。
