Sutor D J, Percival J M, Doonan S
Br J Urol. 1979 Aug;51(4):253-5. doi: 10.1111/j.1464-410x.1979.tb04703.x.
Normal urine was examined for substances which inhibited the formation of calcium oxalate, using a separation scheme which involved ultrafiltration, alcohol precipitation, electrophoresis and paper chromatography. The major anionic inhibitors were found to be citric acid, isocitric acid and pyrophosphate. The inhibitory activity of urine and that of an artificial urine having the same concentration of urea, creatinine, sodium, potassiu, magnesium, calcium, ammonia, sulphate, chloride, inorganic phosphate, oxalate, citrate, isocitrate and pyrophosphate, and the same pH were identical. This confirmed that urine does not contain an unidentified potent substance which inhibits the formation of calcium oxalate.
采用涉及超滤、乙醇沉淀、电泳和纸色谱法的分离方案，对正常尿液中的抑制草酸钙形成的物质进行了检测。结果发现主要的阴离子抑制剂为柠檬酸、异柠檬酸和焦磷酸盐。尿液以及含有相同浓度尿素、肌酐、钠、钾、镁、钙、氨、硫酸盐、氯化物、无机磷酸盐、草酸盐、柠檬酸盐、异柠檬酸盐和焦磷酸盐且pH相同的人工尿液的抑制活性是相同的。这证实了尿液中不含有未鉴定出的强效抑制草酸钙形成的物质。
