Faculty of Medicine, Department of Physiology, Hacettepe University, Ankara, Turkey.
Clin Hemorheol Microcirc. 2011;47(1):45-52. doi: 10.3233/CH-2010-1364.
Acrylamide which is formed via reaction of reducing sugars with amino acids during food processing at high temperatures is not only neurotoxic and carcinogenic, but it also damages erythrocyte membrane and generates micronucleated erythrocytes. In the present study, effects of chronic administration of acrylamide at a dose which does not induce neurotoxicity were evaluated on blood viscosity parameters (hematocrit, erythrocyte deformability, erythrocyte aggregation and plasma viscosity). Twenty adult male Sprague-Dawley rats were divided into control and acrylamide groups. The acrylamide group received 10 mg/kg/day acrylamide, whereas the control group received saline (vehicle), both in 10 ml/kg/day volume via gastric gavage. Erythrocyte aggregation and deformability were measured with LORCA and plasma viscosity with cone-plate viscometer. Erythrocyte deformability was measured before, and at the end of the 3rd and the 5th weeks of acrylamide administration. Hematocrit, erythrocyte aggregation and plasma viscosity were measured only at the end of the 5th week. Acrylamide caused a significant decrease in the deformability index of erythrocytes (at the end of the 3rd week, control: 0.606 ± 0.003, acrylamide: 0.595 ± 0.003, p < 0.05) (at the end of the 5th week, control: 0.606 ± 0.002, acrylamide: 0.588 ± 0.002, p < 0.01). Aggregation tendency and plasma viscosity were slightly higher in the acrylamide group, however the difference was not statistically significant. These results imply that acrylamide which does not cause neurotoxicity in rats may alter blood viscosity if chronically taken.
丙烯酰胺是在食品加工过程中高温下还原糖与氨基酸反应形成的，不仅具有神经毒性和致癌性，还会损害红细胞膜并产生微核红细胞。在本研究中，评估了在不会引起神经毒性的剂量下，长期给予丙烯酰胺对血液粘度参数（红细胞压积、红细胞变形性、红细胞聚集和血浆粘度）的影响。将 20 只成年雄性 Sprague-Dawley 大鼠分为对照组和丙烯酰胺组。丙烯酰胺组每天给予 10mg/kg 丙烯酰胺，对照组给予生理盐水（载体），均以 10ml/kg/天的体积通过胃管给药。用 LORCA 测量红细胞聚集和变形性，用锥板粘度计测量血浆粘度。在丙烯酰胺给药前、第 3 周和第 5 周末测量红细胞变形性。仅在第 5 周末测量红细胞压积、红细胞聚集和血浆粘度。丙烯酰胺导致红细胞变形性指数显著下降（第 3 周末，对照组：0.606±0.003，丙烯酰胺组：0.595±0.003，p<0.05）（第 5 周末，对照组：0.606±0.002，丙烯酰胺组：0.588±0.002，p<0.01）。丙烯酰胺组的聚集倾向和血浆粘度略有升高，但差异无统计学意义。这些结果表明，丙烯酰胺在大鼠中不会引起神经毒性，如果长期摄入，可能会改变血液粘度。
