安卡福凝血栓对红细胞聚集的影响：血液流变学研究。

Effects of Ankaferd Hemostat on red blood cell aggregation: a hemorheological study.

出版信息

Turk J Med Sci. 2019 Feb 11;49(1):356-360. doi: 10.3906/sag-1808-60.

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7350838/

Abstract

BACKGROUND/AIM: Ankaferd hemostat (ABS; Ankaferd blood stopper, İstanbul, Turkey) is a prohemostatic agent affecting erythrocytes. The hemostatic action of ABS depends upon fibrinogen gamma chain, prothrombin, and red blood cells. The aim of this study was to assess the effects of ABS on erythrocyte aggregation via hemorheological analyses.

MATERIALS AND METHODS

To measure erythrocyte aggregation, blood samples were obtained from healthy, nonsmoker volunteers who had not taken any medication in the previous 10 days. One mL of blood was placed into the laser-assisted optical rotational cell analyzer (LORCA), into the chamber formed by the gap between two concentric glass cylinders. The solution prepared with ABS and saline was added to blood in incremental amounts of 10 μL, 20 μL, 30 μL, 40 μL, 50 μL, 60 μL, 70 μL, and 100 μL. Erythrocyte aggregation was determined by laser-assisted optical rotational cell analyzer at 37 °C

RESULTS

AMPwas found to be 17.7 ± 2.1 au in the blood without ABS, whereas it was lower in the blood with ABS. AMP was 16.0 ± 3.3 in the ABS-added blood group. RBC aggregates did not form faster when cells contacted ABS. The t t½ value was 4.6 ± 2.6 in the ABS-added blood group and 1.9 ± 0.20 in the control group. Aggregation was faster in the control group (P = 0.03). AI, which is a combination of AMP and t½, was lowered in the ABS group (48.7 ± 12.3) compared to the control group (65.8 ± 1.6) (P = 0.02). It was notable that the γIsc max (sec-1) value of the control was higher (200 ± 106) than the ABS-added blood group (141 ± 51.0).

CONCLUSION

ABS has antierythroid aggregation effect. ABS inhibits pathological aggregation of red blood cells. Antithrombotic clinical effects of ABS may be ascribed to the antierythroid aggregan actions of the drug.

摘要

背景/目的：安可福止血剂（ABS；Ankaferd 血液止血剂，伊斯坦布尔，土耳其）是一种影响红细胞的促凝血剂。ABS 的止血作用取决于纤维蛋白原 γ 链、凝血酶原和红细胞。本研究旨在通过血液流变学分析评估 ABS 对红细胞聚集的影响。

材料和方法

为了测量红细胞聚集，从 10 天内未服用任何药物的健康、不吸烟的志愿者中采集 1ml 血液。将 1ml 血液放入激光辅助光学旋转细胞分析仪（LORCA）中，放入两个同心玻璃圆柱之间形成的间隙的腔室中。将用 ABS 和生理盐水制备的溶液以 10μL、20μL、30μL、40μL、50μL、60μL、70μL 和 100μL 的增量添加到血液中。在 37°C 下通过激光辅助光学旋转细胞分析仪测定红细胞聚集。

结果

在没有 ABS 的血液中，AMP 为 17.7±2.1au，而在有 ABS 的血液中则较低。在添加 ABS 的血液组中，AMP 为 16.0±3.3。当细胞接触 ABS 时，RBC 聚集物不会更快地形成。在添加 ABS 的血液组中，t t½ 值为 4.6±2.6，在对照组中为 1.9±0.20。在对照组中，聚集速度更快（P=0.03）。在 ABS 组中，AI（AMP 和 t t½ 的组合）降低（48.7±12.3），而在对照组中升高（65.8±1.6）（P=0.02）。值得注意的是，对照组的γIsc max（sec-1）值较高（200±106），而添加 ABS 的血液组较低（141±51.0）。