在热带假丝酵母中通过衰减细胞内木酮糖脱氢酶活性来提高木糖醇的产量。

Enhancement of xylitol production by attenuation of intracellular xylitol dehydrogenase activity in Candida tropicalis.

机构信息

Higher Education Center for Bioregulator Research, Chonnam National University, Gwangju, 500-757, Korea.

出版信息

Biotechnol Lett. 2011 Jun;33(6):1209-13. doi: 10.1007/s10529-011-0558-z. Epub 2011 Feb 18.

Abstract

To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l(-1) h(-1), respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production.

摘要

为了构建能够高产木糖醇且无需共底物的热带假丝酵母菌株，我们对酵母进行了弱化木酮糖脱氢酶（XDH）的工程改造，然后评估了木糖醇生产的效率。突变株 XDH-5（只有一个 XDH 基因拷贝）和 ARSdR-16（XDH 基因突变）的活性分别为野生型（WT）XDH 的 70%和 40%。WT、XDH-5 和 ARSdR-16 将木糖转化为木糖醇的转化率分别为 62%、64%和 75%，相应的生产率分别为 0.52、0.54 和 0.62 g l(-1) h(-1)。ARSdR-16 突变株在简单的生产过程中以高产率和高生产率生产木糖醇，不需要甘油等共底物。该菌株为高效、经济地生产木糖醇提供了有前途的替代方案。