Higher Education Center for Bioregulator Research, Chonnam National University, Gwangju, 500-757, Korea.
Biotechnol Lett. 2011 Jun;33(6):1209-13. doi: 10.1007/s10529-011-0558-z. Epub 2011 Feb 18.
To construct Candida tropicalis strains that produce a high yield of xylitol with no requirement for co-substrates, we engineered the yeast with an attenuated xylitol dehydrogenase (XDH) and then assessed the efficiency of xylitol production The mutants, strains XDH-5 (with only one copy of the XDH gene), and ARSdR-16 (with a mutated XDH gene) showed 70 and 40% of wild type (WT) XDH activity, respectively. Conversions of xylose to xylitol by WT, XDH-5, and ARSdR-16 were 62, 64, and 75%, respectively, with productivities of 0.52, 0.54, and 0.62 g l(-1) h(-1), respectively. The ARSdR-16 mutant strain produced xylitol with high yield and high productivity in a simple process that required no co-substrates, such as glycerol. This strain represents a promising alternative for efficient and cost-effective xylitol production.
为了构建能够高产木糖醇且无需共底物的热带假丝酵母菌株，我们对酵母进行了弱化木酮糖脱氢酶（XDH）的工程改造，然后评估了木糖醇生产的效率。突变株 XDH-5（只有一个 XDH 基因拷贝）和 ARSdR-16（XDH 基因突变）的活性分别为野生型（WT）XDH 的 70%和 40%。WT、XDH-5 和 ARSdR-16 将木糖转化为木糖醇的转化率分别为 62%、64%和 75%，相应的生产率分别为 0.52、0.54 和 0.62 g l(-1) h(-1)。ARSdR-16 突变株在简单的生产过程中以高产率和高生产率生产木糖醇，不需要甘油等共底物。该菌株为高效、经济地生产木糖醇提供了有前途的替代方案。
