Heuer Wieland, Kettenring Andreas, Demling Anton, Stumpp Sascha Nico, Gellermann Eva, Winkel Andreas, Stiesch Meike
1 Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Germany.
J Oral Implantol. 2013 Dec;39(6):648-54. doi: 10.1563/AAID-JOI-D-10-00088. Epub 2011 Feb 18.
One of the principal problems in oral implantation is inflammation of peri-implant hard and soft tissues caused by bacterial biofilms. The purpose of the present study was to evaluate the microbial diversity of peri-implant biofilms on 2 different implant-anchored attachment types in vivo. Samples of peri-implant sulcus fluid were collected from 8 patients with implant-supported bar attachments and 8 patients with implant-anchored telescopic double crown attachments. Samples of sulcus fluid of the adjacent teeth were also collected from the partially edentulous patients with implant fixed telescopic double crowns. The mixed amplicons of 16S rRNA fragments of different bacterial origins were separated by use of single-strand conformation polymorphism analysis to identify the predominant bacterial genera. With 3.5 ± 2.1 different predominant bacterial genera in the sulcus fluid surrounding implant-supported bar attachments and 6.3 ± 3.1 different predominant genera in the sulcular fluid of implant-anchored double crown attachments, the differences were not statistically significant (P = .11). The microbial diversity in the sulcus fluid surrounding the remaining dentition was similar to that of the implant fixed telescopic attachments (6.3 ± 2.1). Aside from host response and other individual factors, the microbial diversity of peri-implant biofilms seems to be impaired by cofactors such as the possibility of cleaning the implant-supported supraconstructions and the different plaque-retaining sites. Nevertheless, these differences do not lead to statistically significant differences in the microbial diversity of peri-implant plaques.
口腔种植的主要问题之一是细菌生物膜引起的种植体周围软硬组织炎症。本研究的目的是评估体内两种不同种植体固位附着类型的种植体周围生物膜的微生物多样性。从8例种植体支持杆附着患者和8例种植体固位套筒冠附着患者中采集种植体周围龈沟液样本。还从部分牙列缺损且有种植体固定套筒冠的患者中采集相邻牙齿的龈沟液样本。利用单链构象多态性分析分离不同细菌来源的16S rRNA片段的混合扩增子，以鉴定主要细菌属。种植体支持杆附着周围龈沟液中有3.5±2.1种不同的主要细菌属，种植体固位双冠龈沟液中有6.3±3.1种不同的主要细菌属，差异无统计学意义（P = 0.11）。剩余牙列周围龈沟液中的微生物多样性与种植体固定套筒冠附着的相似（6.3±2.1）。除宿主反应和其他个体因素外，种植体周围生物膜的微生物多样性似乎受到诸如清洁种植体支持的上部结构的可能性和不同菌斑滞留部位等辅助因素的影响。然而，这些差异并未导致种植体周围菌斑微生物多样性的统计学显著差异。
