Leuven BIOMAT Research Cluster, Department of Conservative Dentistry, School of Dentistry, Oral Pathology and Maxillo-Facial Surgery, Catholic University of Leuven, Kapucijnenvoer 7, B-3000 Leuven, Belgium.
J Dent. 2011 May;39(5):351-60. doi: 10.1016/j.jdent.2011.02.005. Epub 2011 Feb 18.
To compare mineral density of residual dentine after excavation with different caries-removal techniques and to evaluate the diagnostic potential of laser-induced fluorescence (LIF), measured by DIAGNOdent, as a tool to determine the caries-removal endpoint.
Carious teeth were excavated by tungsten-carbide round burs (Komet), ceramic burs (CeraBurs, Komet), sono-abrasion (Cariex TC tips, Kavo), and by chemo-mechanical excavation using two enzyme-based solutions (exp. SFC-V and SFC-VIII, 3M-ESPE) or a sodium hypochlorite-based solution (Carisolv, MediTeam). The caries-excavated teeth were scanned by micro-CT (1172, Skyscan), after which the mineral density at the bottom dentine was correlated to LIF measurements at the same region. A micro-CT threshold for dentine caries was defined by comparison with surface-hardness measurements. The intensity of dentine staining was evaluated by analysing the component 'L*' in CIE-Lab-converted images from the excavated teeth.
No statistically significant difference in mineral density was found at the bottom of the cavities prepared with the different caries-excavation techniques, except for exp. SFC-V that left residual dentine with a significantly higher mineral density than when CeraBurs were used (Tukey-Kramer, p<0.05). Absence of residual caries was associated with darker staining of dentine. No significant correlation was found between the distance from the deepest cavity point to the pulp-chamber roof and LIF measurements. A strong negative correlation (R=-0.86, p<0.01) was however found between L* values and LIF measurements, indicating that staining in residual dentine leads to higher LIF measurements.
LIF measured by DIAGNOdent is influenced by staining in residual dentine. Therefore, its use to determine the caries-removal endpoint is doubtful.
比较不同龋蚀去除技术后残馀牙本质的矿物质密度，并评估激光诱导荧光（LIF）的诊断潜力，通过 DIAGNOdent 测量，作为确定龋蚀去除终点的工具。
用碳化钨圆锯（Komet）、陶瓷锯（CeraBurs，Komet）、超声喷砂（Cariex TC 尖端，Kavo）以及两种基于酶的溶液（实验 SFC-V 和 SFC-VIII，3M-ESPE）或次氯酸钠溶液（Carisolv，MediTeam）进行化学机械去龋。用 micro-CT（1172，Skyscan）扫描去龋后的牙齿，然后将底部牙本质的矿物质密度与同一区域的 LIF 测量值相关联。通过与表面硬度测量值比较，定义了牙本质龋的 micro-CT 阈值。通过分析从去龋牙齿的 CIE-Lab 转换图像中“L*”分量，评估牙本质染色的强度。
除实验 SFC-V 外，用不同龋蚀去除技术制备的窝洞底部的矿物质密度没有统计学差异，实验 SFC-V 组的矿物质密度明显高于 CeraBurs 组（Tukey-Kramer，p<0.05）。没有残留龋的存在与牙本质染色较暗有关。从最深的窝洞点到牙髓腔顶的距离与 LIF 测量值之间没有显著相关性。然而，L*值与 LIF 测量值之间存在很强的负相关（R=-0.86，p<0.01），表明残馀牙本质中的染色导致更高的 LIF 测量值。
DIAGNOdent 测量的 LIF 受残馀牙本质染色的影响。因此，其用于确定龋蚀去除终点的用途值得怀疑。
