大肠杆菌山梨醇操纵子中gutQ基因的核苷酸序列及表达
Nucleotide sequence and expression of the gutQ gene within the glucitol operon of Escherichia coli.
作者信息
Yamada M, Yamada Y, Saier M H
机构信息
Department of Biology, University of California, San Diego, La Jolla 92093.
出版信息
DNA Seq. 1990;1(2):141-5. doi: 10.3109/10425179009016042.
The glucitol operon in Escherichia coli is known to consist of five structural genes with the order: gut O P A B D M R. We have sequenced downstream from gutR and have identified an open reading frame encoding a water-soluble protein (223 amino acids; molecular weight = 23,562) with a putative ATP binding site. Expression of this protein in a maxicell system has been demonstrated. A repetitive extragenic palindromic (REP) sequence capable of forming stem-loop structures follows gutQ in the downstream, presumptive intercistronic region. The function of the Gut Q protein is not known.
已知大肠杆菌中的葡糖醇操纵子由五个结构基因组成，顺序为：gut O P A B D M R。我们对gutR下游进行了测序，并鉴定出一个开放阅读框，其编码一种具有假定ATP结合位点的水溶性蛋白质（223个氨基酸；分子量 = 23,562）。已证实在一个大细胞系统中表达了这种蛋白质。在下游假定的基因间区域，gutQ之后有一个能够形成茎环结构的重复基因外回文（REP）序列。Gut Q蛋白的功能尚不清楚。
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