Division of Pathology, Department of Oral Diagnostic Sciences, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Exp Cell Res. 2013 May 1;319(8):1220-8. doi: 10.1016/j.yexcr.2013.03.004. Epub 2013 Mar 13.
Cancer stem cells (CSCs) are among the target cells of cancer therapy because they are uniquely involved in both cancer progression and sensitivity to chemotherapeutic agents. We identified side population (SP) cells, which are known to be an enriched population of CSC, in five oral squamous cell carcinoma (OSCC) cells (SCC9, SCC25, TOSCC7, TOSCC17, and TOSCC23). The percentages of SP cells ranged from 0% to 3.3%, with TOSCC23 cells showing the highest percentages of SP cells (3.3% of the total cell population). The SP cells isolated from TOSCC23 cells also showed greater cell proliferation and invasion compared to non-SP (MP) cells. Therefore, our initial findings suggested that SP cells were enriched for CSC-like cells. Furthermore, DNA microarray analysis revealed that the expression of cell proliferation-related and anti-apoptotic genes was greater in SP cells compared to MP cells. We focused on Lin28a, which showed the highest expression (approximately 22-fold) among the upregulated genes. The overexpression of Lin28a in TOSCC23 cells increased their proliferation, colony formation, and invasion. These findings suggest that Lin28a is an appropriate CSC target molecule for OSCC treatment.
癌症干细胞（CSC）是癌症治疗的靶细胞之一，因为它们独特地参与癌症的进展和对化疗药物的敏感性。我们在五种口腔鳞状细胞癌（OSCC）细胞（SCC9、SCC25、TOSCC7、TOSCC17 和 TOSCC23）中鉴定了侧群（SP）细胞，已知其为 CSC 的富集群体。SP 细胞的比例从 0%到 3.3%不等，TOSCC23 细胞显示出最高的 SP 细胞比例（总细胞群的 3.3%）。从 TOSCC23 细胞中分离的 SP 细胞也表现出比非 SP（MP）细胞更高的细胞增殖和侵袭能力。因此，我们的初步研究结果表明，SP 细胞富含 CSC 样细胞。此外，DNA 微阵列分析显示，与 MP 细胞相比，SP 细胞中与细胞增殖相关和抗凋亡基因的表达更高。我们关注 Lin28a，它在上调基因中表达最高（约 22 倍）。在 TOSCC23 细胞中过表达 Lin28a 会增加其增殖、集落形成和侵袭。这些发现表明 Lin28a 是治疗 OSCC 的合适 CSC 靶分子。
