DNA 中 5-羟甲基胞嘧啶的甲基转移酶导向衍生化。
Methyltransferase-directed derivatization of 5-hydroxymethylcytosine in DNA.
机构信息
Department of Biological DNA Modification, Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241 Vilnius, Lithuania.
出版信息
Angew Chem Int Ed Engl. 2011 Feb 25;50(9):2090-3. doi: 10.1002/anie.201007169. Epub 2011 Jan 26.
Abstract
Cytosine modification by AdoMet–dependent DNA methyltransferases is part of an epigenetic regulatory network in vertebrates. Here we show that, in the absence of AdoMet, bacterial cytosine-5 methyltransferases can catalyze condensation of aliphatic thiols and selenols to 5-hydroxymethylcytosine in DNA yielding 5-chalcogenomethyl derivatives. These new atypical reactions open new ways for sequence-specific derivatization and analysis of 5-hydroxymethylcytosine, a recently discovered nucleobase in mammalian DNA
摘要
依赖 AdoMet 的 DNA 甲基转移酶对胞嘧啶的修饰是脊椎动物表观遗传调控网络的一部分。在这里，我们表明，在没有 AdoMet 的情况下，细菌胞嘧啶-5 甲基转移酶可以催化脂肪族硫醇和硒醇与 DNA 中的 5-羟甲基胞嘧啶缩合，生成 5-巯基甲基和 5-硒基甲基衍生物。这些新的非典型反应为 5-羟甲基胞嘧啶的序列特异性衍生化和分析开辟了新的途径，5-羟甲基胞嘧啶是最近在哺乳动物 DNA 中发现的一种核苷酸碱基。
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