Department of Biological DNA Modification, Institute of Biotechnology, Vilnius University, V. A. Graičiūno 8, 02241 Vilnius, Lithuania.
Angew Chem Int Ed Engl. 2011 Feb 25;50(9):2090-3. doi: 10.1002/anie.201007169. Epub 2011 Jan 26.
Cytosine modification by AdoMet–dependent DNA methyltransferases is part of an epigenetic regulatory network in vertebrates. Here we show that, in the absence of AdoMet, bacterial cytosine-5 methyltransferases can catalyze condensation of aliphatic thiols and selenols to 5-hydroxymethylcytosine in DNA yielding 5-chalcogenomethyl derivatives. These new atypical reactions open new ways for sequence-specific derivatization and analysis of 5-hydroxymethylcytosine, a recently discovered nucleobase in mammalian DNA
依赖 AdoMet 的 DNA 甲基转移酶对胞嘧啶的修饰是脊椎动物表观遗传调控网络的一部分。在这里，我们表明，在没有 AdoMet 的情况下，细菌胞嘧啶-5 甲基转移酶可以催化脂肪族硫醇和硒醇与 DNA 中的 5-羟甲基胞嘧啶缩合，生成 5-巯基甲基和 5-硒基甲基衍生物。这些新的非典型反应为 5-羟甲基胞嘧啶的序列特异性衍生化和分析开辟了新的途径，5-羟甲基胞嘧啶是最近在哺乳动物 DNA 中发现的一种核苷酸碱基。
