Ji Debin, Lin Krystal, Song Jikui, Wang Yinsheng
Department of Chemistry, University of California, Riverside, CA 92521, USA.
Mol Biosyst. 2014 Jul;10(7):1749-52. doi: 10.1039/c4mb00150h. Epub 2014 Apr 30.
We investigated systematically the effects of Tet-induced oxidation products of 5-methylcytosine on Dnmt1- and DNMT3a-mediated cytosine methylation in synthetic duplex DNA. We found that the replacement of 5-methylcytosine at a CpG site with a 5-hydroxymethylcytosine, 5-formylcytosine, 5-carboxylcytosine or 5-hydroxymethyluracil resulted in altered methylation of cytosine at both the opposite and the neighboring CpG sites. Our results provided important new knowledge about the implications of the 5-methylcytosine oxidation products in maintenance cytosine methylation.
我们系统地研究了四氢叶酸诱导的5-甲基胞嘧啶氧化产物对合成双链DNA中Dnmt1和DNMT3a介导的胞嘧啶甲基化的影响。我们发现，在CpG位点用5-羟甲基胞嘧啶、5-甲酰基胞嘧啶、5-羧基胞嘧啶或5-羟甲基尿嘧啶取代5-甲基胞嘧啶会导致相对和相邻CpG位点的胞嘧啶甲基化发生改变。我们的结果为5-甲基胞嘧啶氧化产物在维持胞嘧啶甲基化中的作用提供了重要的新知识。
