State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People's Republic of China.
Appl Biochem Biotechnol. 2011 Aug;164(7):1215-24. doi: 10.1007/s12010-011-9207-1. Epub 2011 Feb 24.
A comparative evaluation of different cell disruption methods for the release of lipids from marine Chlorella vulgaris cells was investigated. The cell growth of C. vulgaris was observed. Lipid concentrations from different disruption methods were determined, and the fatty acid composition of the extracted lipids was analyzed. The results showed that average productivity of C. vulgaris biomass was 208 mg L⁻¹ day⁻¹. The lipid concentrations of C. vulgaris were 5%, 6%, 29%, 15%, 10%, 7%, 22%, 24%, and 18% when using grinding with quartz sand under wet condition, grinding with quartz sand under dehydrated condition, grinding in liquid nitrogen, ultrasonication, bead milling, enzymatic lysis by snailase, enzymatic lysis by lysozyme, enzymatic lysis by cellulose, and microwaves, respectively. The shortest disruption time was 2 min by grinding in liquid nitrogen. The unsaturated and saturated fatty acid contents of C. vulgaris were 71.76% and 28.24%, respectively. The extracted lipids displayed a suitable fatty acid profile for biodiesel [C16:0 (23%), C16:1 (23%), and C18:1 (~45%)]. Overall, grinding in liquid nitrogen was identified as the most effective method in terms of disruption efficiency and time.
对不同的细胞破碎方法从海洋小球藻细胞中释放脂质进行了比较评价。观察了 C. vulgaris 的细胞生长。测定了不同破碎方法的脂质浓度，并分析了提取脂质的脂肪酸组成。结果表明，C. vulgaris 生物质的平均生产力为 208mg L ⁻¹ 天 ⁻¹ 。当使用湿条件下用石英砂研磨、脱水条件下用石英砂研磨、在液氮中研磨、超声、珠磨、蜗牛酶酶解、溶菌酶酶解、纤维素酶酶解和微波时，C. vulgaris 的脂质浓度分别为 5%、6%、29%、15%、10%、7%、22%、24%和 18%。通过液氮研磨达到最短的破碎时间为 2 分钟。C. vulgaris 的不饱和和饱和脂肪酸含量分别为 71.76%和 28.24%。提取的脂质显示出适合生物柴油的脂肪酸谱[C16:0（23%）、C16:1（23%）和 C18:1（~45%）]。总的来说，从破碎效率和时间来看，液氮研磨被确定为最有效的方法。
