Chieffi P P, Ueda M, Camargo E D, de Souza A M, Guedes M L, Gerbi L J, Spir M, Moreira A S
Instituto de Medicina Tropical de São Paulo, Brasil.
Rev Inst Med Trop Sao Paulo. 1990 May-Jun;32(3):204-10. doi: 10.1590/s0036-46651990000300010.
An enzyme-linked immunosorbent assay (ELISA), employing antigens from Toxocara canis larvae and the absortion of suspected sera with Ascaris lumbricoides extracts was used in a seroepidemiological study performed in five municipalities of São Paulo State, Brazil (São Paulo, Campinas, Santos, Marília and Presidente Prudente) in order to determine the frequency of antibodies to Toxocara. In 2,025 blood samples collected, 806 proceeded from male subjects and 1,219 from females; 483 samples were collected from subjects under 15 years of age and the remaining 1,542 from subjects aged 15 years or over. Among the 2,025 sera investigated, 3.60% had antibodies to Toxocara at significant levels. A moderate predominance of infection with Toxocara among male subjects (3.72%) was observed, although the difference was not statistically significant when this rate was compared with that for female (3.28%). Related to age, a higher frequency of positive results was detected among subjects under 15 years (6.41%) against the older group (2.53%). A trend of more elevated rates of infection was observed in municipalities with high demographic densities (São Paulo, Campinas and Santos). Nevertheless, such findings only appeared to be statistically significant in subjects younger than 15 years.
在巴西圣保罗州的五个城市（圣保罗、坎皮纳斯、桑托斯、马林利亚和普鲁登特总统城）进行的一项血清流行病学研究中，采用了一种酶联免疫吸附测定法（ELISA），该方法使用犬弓首蛔虫幼虫的抗原以及用蛔虫提取物吸收疑似血清，以确定抗弓首蛔虫抗体的频率。在所采集的2025份血样中，806份来自男性受试者，1219份来自女性；483份样本采集自15岁以下的受试者，其余1542份来自15岁及以上的受试者。在被调查的2025份血清中，3.60%含有显著水平的抗弓首蛔虫抗体。尽管将男性受试者（3.72%）的感染率与女性（3.28%）的感染率相比较时差异无统计学意义，但观察到男性受试者中弓首蛔虫感染略占优势。与年龄相关，15岁以下的受试者（6.41%）的阳性结果频率高于年龄较大的组（2.53%）。在人口密度高的城市（圣保罗、坎皮纳斯和桑托斯）观察到感染率有升高的趋势。然而，这些发现仅在15岁以下的受试者中似乎具有统计学意义。
