Allergy Service, Hospital General de Calde, Lugo, Spain.
Ann Allergy Asthma Immunol. 2011 Mar;106(3):223-9. doi: 10.1016/j.anai.2010.11.014. Epub 2010 Dec 23.
Seafood allergy has been related to mite sensitization, mainly mediated by the muscle protein tropomyosin.
To determine the correlation between seafood hypersensitivity and mite sensitization (Dermatophagoides pteronyssinus and Chortoglyphus arcuatus, a highly prevalent storage mite in Spain) and to investigate the implication of tropomyosin in cross-reactivity.
Patients from Northwest Spain were divided into 2 groups. The mite-seafood group contained 30 allergic mite individuals with a clinical history of food hypersensitivity. The mite group contained 40 individuals with positive skin prick test results to D pteronyssinus and C arcuatus but negative seafood test results. Specific IgE (sIgE) to whole mite and shrimp extracts, mite tropomyosin (rDer p 10), and shrimp tropomyosin (rPen a 1) were determined in each serum sample. Allergenic profiles were analyzed by immunoblot. Cross-reactivity studies were investigated by enzyme-linked immunosorbent assay and immunoblot inhibition studies.
In the mite-seafood group, 71% of patients had positive sIgE results to shrimp and 55% of them to shrimp tropomyosin. A strong correlation was found between sIgE to shrimp tropomyosin and mite tropomyosin. Positive correlation was observed between sIgE to shrimp tropomyosin and severity of symptoms. In the mite group, none of the 20% of patients with sIgE to shrimp tested positive to shrimp tropomyosin. In the immunoblot inhibition experiment, the shrimp extract was totally inhibited by mite extract. These data suggest that primary sensitization is related to mite sensitization.
Tropomyosin does not seem to be the main allergen involved in mite-seafood sensitization in mite sensitized individuals. High levels of sIgE to tropomyosin seem to be related to severity of symptoms.
海鲜过敏与螨致敏有关，主要由肌肉蛋白原肌球蛋白介导。
确定海鲜过敏与螨致敏（在西班牙高度流行的贮藏螨屋尘螨和粗脚粉螨）之间的相关性，并研究原肌球蛋白在交叉反应中的作用。
来自西班牙西北部的患者被分为 2 组。螨-海鲜组包含 30 名有食物过敏史的过敏螨个体。螨组包含 40 名对屋尘螨和粗脚粉螨皮试阳性但海鲜试验阴性的个体。每个血清样本中均测定了对整个螨和虾提取物、螨原肌球蛋白（rDer p 10）和虾原肌球蛋白（rPen a 1）的特异性 IgE（sIgE）。通过免疫印迹分析过敏原谱。通过酶联免疫吸附试验和免疫印迹抑制研究进行交叉反应研究。
在螨-海鲜组中，71%的患者对虾 sIgE 阳性，55%的患者对虾原肌球蛋白 sIgE 阳性。发现虾原肌球蛋白 sIgE 与螨原肌球蛋白 sIgE 之间存在很强的相关性。虾原肌球蛋白 sIgE 与症状严重程度之间存在正相关关系。在螨组中，对虾 sIgE 阳性的 20%患者中无一例对虾原肌球蛋白 sIgE 阳性。在免疫印迹抑制实验中，虾提取物被螨提取物完全抑制。这些数据表明，初次致敏与螨致敏有关。
在螨致敏个体中，原肌球蛋白似乎不是螨-海鲜致敏的主要过敏原。高水平的原肌球蛋白 sIgE 似乎与症状严重程度有关。
