在鼠淋巴结中独特的祖细胞发育为 CD127+NK 细胞：依赖于胸腺和不依赖于胸腺的途径。
Unique progenitors in mouse lymph node develop into CD127+ NK cells: thymus-dependent and thymus-independent pathways.
机构信息
Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC.
出版信息
Blood. 2011 Apr 14;117(15):4012-21. doi: 10.1182/blood-2010-07-298901. Epub 2011 Feb 25.
A subset of natural killer (NK) cells in normal mouse lymph node (LN) expresses CD127 (IL-7 receptor-α chain) and is thought to derive from the thymus. However, CD127(+) NK cells are found in the LN of athymic mice. Therefore, the origin of CD127(+) NK cells in the LN is unclear. Here, we have identified unique NK-cell progenitors (NKPs) in the LN that express the pan-NK cell marker CD49b and CD127 but lack CD122 and lineage markers. The LN NKPs develop in vitro into CD127(+) NK cells that display natural cytotoxicity and cytokine production capacity. They also become CD127(+) NK cells in lymphopenic mice that received a transplant. LN NKPs can be divided into stem cell antigen-1 (Sca-1)(hi) and Sca-1(lo) subsets. The latter comprise ∼ 60% of LN NKPs in normal mouse and < 10% of athymic mouse LN NKPs. Whereas both Sca-1(hi) and Sca-1(lo) NKPs develop into CD127(+) NK cells in vitro, only those derived from Sca-1(lo) LN NKPs have rearranged TCRγ genes. Thus, CD127(+) NK cells in the LN seem to be generated, at least in part, from both thymus-dependent Sca-1(lo) and thymus-independent Sca-1(hi) LN NKPs.
正常小鼠淋巴结（LN）中的一组自然杀伤（NK）细胞表达 CD127（IL-7 受体-α 链），被认为来源于胸腺。然而，无胸腺小鼠的 LN 中也存在 CD127(+) NK 细胞。因此，LN 中 CD127(+) NK 细胞的来源尚不清楚。在这里，我们在 LN 中鉴定出一种独特的 NK 细胞前体（NKP），它表达泛 NK 细胞标记物 CD49b 和 CD127，但缺乏 CD122 和谱系标记物。LN NKP 在体外发育为 CD127(+) NK 细胞，具有自然细胞毒性和细胞因子产生能力。它们也成为接受移植的淋巴减少小鼠中的 CD127(+) NK 细胞。LN NKP 可分为干细胞抗原-1（Sca-1）（高）和 Sca-1（低）亚群。后者约占正常小鼠 LN NKP 的 60%，而无胸腺小鼠 LN NKP 的<10%。虽然 Sca-1（高）和 Sca-1（低）NKP 均可在体外发育为 CD127(+) NK 细胞，但只有来自 Sca-1（低）LN NKP 的细胞具有重排的 TCRγ 基因。因此，LN 中的 CD127(+) NK 细胞似乎至少部分来自于依赖于胸腺的 Sca-1（低）和不依赖于胸腺的 Sca-1（高）LN NKP。
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