Enteric Pathogen Research Laboratory, Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland.
Food Microbiol. 2011 May;28(3):426-33. doi: 10.1016/j.fm.2010.10.003. Epub 2010 Oct 21.
Campylobacter enteritis is a zoonosis, an infectious disease transmissible under normal conditions from vertebrate animals to man, presenting a major global public health burden. In this study, Pulsed Field Gel Electrophoresis (PFGE) was employed to identify common genotypes in a collection of 600 Campylobacter isolates in order to investigate if profiles obtained from retail samples of foodstuffs matched genotypes causing illness in the community in Ireland. The Campylobacters were isolated from retail foodstuffs, and cases of gastroenteritis, over the same 20-month period in three population centres in Ireland. The major observation made was of a high level of PFGE-genotype heterogeneity; 236 SmaI discrete genotypes were found in 507 strains successfully analysed. Analysis of the PFGE profiles revealed 22 common profiles amongst food isolates and those causing enteritis in humans. These cojoint PFGE genotypes indicate that 56 (38%) of the human clinical isolates are genetically related to 129 (36%) of the food isolates. The identification of these recurrent PFGE types, in the sampled Campylobacter coli and Campylobacter jejuni populations, indicates that a high proportion of Campylobacter isolates found in foods of animal origin also occur in patients with symptoms of enteritis. This data adds weight to the epidemiological hypothesis that a high proportion of human Campylobacter cases are contracted via the handling and consumption of contaminated foodstuffs, in particular poultry.
空肠弯曲菌肠炎是一种人畜共患病，在正常条件下，这种传染病可以从脊椎动物传播给人类，给全球公共卫生带来了重大负担。在这项研究中，我们使用脉冲场凝胶电泳（PFGE）来鉴定 600 株空肠弯曲菌分离株中的常见基因型，以调查从爱尔兰社区零售食品样本中获得的图谱是否与引起疾病的基因型相匹配。这些弯曲菌是从零售食品以及在爱尔兰三个人群中心的 20 个月期间的肠胃炎病例中分离出来的。主要的观察结果是高水平的 PFGE 基因型异质性；在成功分析的 507 株菌株中发现了 236 个 SmaI 离散基因型。PFGE 图谱分析显示，在食品分离株和引起人类肠炎的分离株中存在 22 种常见图谱。这些共同的 PFGE 基因型表明，56（38%）例人类临床分离株与 129（36%）例食品分离株在遗传上有关。在采样的空肠弯曲菌和空肠弯曲菌群体中鉴定出这些反复出现的 PFGE 类型，表明在动物源性食品中发现的空肠弯曲菌分离株的很大一部分也存在于有肠炎症状的患者中。这一数据为流行病学假说提供了更多证据，即很大一部分人类弯曲菌病例是通过处理和食用受污染的食物，特别是家禽而感染的。
