Institut des Nutraceutiques et des Aliments Fonctionnels, Université Laval, Québec, Québec G1K 7P4, Canada.
Food Microbiol. 2011 May;28(3):568-72. doi: 10.1016/j.fm.2010.11.012. Epub 2010 Nov 24.
This study was conducted to evaluate the inactivation of murine norovirus (MNV-1) and hepatitis A virus (HAV) by pulsed ultraviolet (UV) light. MNV-1 was used as a model for human norovirus. Viral suspensions of about 10(6) PFU/ml were exposed to pulses of UV light for different times and at different distances in a Xenon Steripulse device (model RS-3000C). Inactivation studies were also carried out on 1-cm(2) stainless steel and polyvinyl chloride disks with 10(5) PFU/ml. Inactivation of MNV-1 and HAV at 10.5 cm from the UV source was greater on inert surfaces than in suspension. The presence of organic matter (fetal bovine serum) reduced the effectiveness of pulsed light both in suspension and on surfaces. However, 2-s treatment in the absence of FBS completely inactivated (5 log reduction) the viral load at different distances tested, whether in suspension (MNV-1) or on disks (MNV-1 and HAV). The same treatment in the presence of fetal bovine serum (5%) allowed a reduction of about 3 log. This study showed that short duration pulses represent an excellent alternative for inactivation of food-borne viruses. This technology could be used to inactivate viruses in drinking water or on food-handling surfaces.
本研究旨在评估脉冲紫外（UV）光对鼠诺如病毒（MNV-1）和甲型肝炎病毒（HAV）的灭活效果。MNV-1 被用作人类诺如病毒的模型。将约 10(6)PFU/ml 的病毒悬浮液暴露于 Xenon Steripulse 设备（型号 RS-3000C）中的 UV 光下不同时间和不同距离的脉冲下。还在 1cm(2)不锈钢和聚氯乙烯盘上进行了 10(5)PFU/ml 的灭活研究。在距 UV 源 10.5cm 处，惰性表面上的 MNV-1 和 HAV 灭活效果优于悬浮液。存在有机物（胎牛血清）会降低脉冲光在悬浮液和表面上的有效性。然而，在无 FBS 的情况下进行 2 秒处理可在不同距离（悬浮液中的 MNV-1 和盘上的 MNV-1 和 HAV）完全灭活病毒载量（5 对数减少）。在存在胎牛血清（5%）的情况下，相同的处理可减少约 3 对数。本研究表明，短持续时间的脉冲是食源性病原体灭活的理想选择。这项技术可用于灭活饮用水或食品处理表面上的病毒。
