涡虫：用于再生、成体干细胞调控、衰老及行为分子研究的通用且强大的模型系统。
Planarians: a versatile and powerful model system for molecular studies of regeneration, adult stem cell regulation, aging, and behavior.
作者信息
Oviedo Néstor J, Nicolas Cindy L, Adams Dany S, Levin Michael
机构信息
Center for Regenerative and Developmental Biology, Forsyth Institute and Developmental Biology Department, Harvard School of Dental Medicine, Boston, MA 02115, USA.
出版信息
CSH Protoc. 2008 Oct 1;2008:pdb.emo101. doi: 10.1101/pdb.emo101.
Abstract
INTRODUCTIONIn recent years, planarians have been increasingly recognized as an emerging model organism amenable to molecular genetic techniques aimed at understanding complex biological tasks commonly observed among metazoans. Growing evidence suggests that this model organism is uniquely poised to inform us about the mechanisms of tissue regeneration, stem cell regulation, tissue turnover, pharmacological action of diverse drugs, cancer, and aging. This article provides an overview of the planarian model system with special attention to the species Schmidtea mediterranea. Additionally, information is provided about the most popular use of this organism, together with modern genomic resources and technical approaches.
摘要
引言
近年来，涡虫越来越被公认为是一种新兴的模式生物，适用于旨在理解后生动物中常见复杂生物学任务的分子遗传学技术。越来越多的证据表明，这种模式生物能独特地为我们提供有关组织再生、干细胞调控、组织更新、各种药物的药理作用、癌症和衰老机制的信息。本文概述了涡虫模式系统，特别关注地中海涡虫这一物种。此外，还提供了关于这种生物最常见用途的信息，以及现代基因组资源和技术方法。
相似文献
1
Planarians: a versatile and powerful model system for molecular studies of regeneration, adult stem cell regulation, aging, and behavior.涡虫：用于再生、成体干细胞调控、衰老及行为分子研究的通用且强大的模型系统。
CSH Protoc. 2008 Oct 1;2008:pdb.emo101. doi: 10.1101/pdb.emo101.
2
Stem cells and the Planarian Schmidtea mediterranea.干细胞与地中海涡虫
C R Biol. 2007 Jun-Jul;330(6-7):498-503. doi: 10.1016/j.crvi.2007.05.005. Epub 2007 Jun 12.
3
Allometric scaling and proportion regulation in the freshwater planarian Schmidtea mediterranea.地中海涡虫（Schmidtea mediterranea）的异速生长缩放与比例调节
Dev Dyn. 2003 Feb;226(2):326-33. doi: 10.1002/dvdy.10228.
4
Schmidtea happens: Re-establishing the planarian as a model for studying the mechanisms of regeneration.再生研究的新模式：让涡虫重现江湖。
Curr Top Dev Biol. 2022;147:307-344. doi: 10.1016/bs.ctdb.2022.01.002. Epub 2022 Feb 25.
5
Smed454 dataset: unravelling the transcriptome of Schmidtea mediterranea.Smed454 数据集：揭示地中海星虫的转录组。
BMC Genomics. 2010 Dec 31;11:731. doi: 10.1186/1471-2164-11-731.
6
A dual platform approach to transcript discovery for the planarian Schmidtea mediterranea to establish RNAseq for stem cell and regeneration biology.采用双平台方法发现扁形动物 Schmidtea mediterranea 的转录本，为干细胞和再生生物学建立 RNAseq。
PLoS One. 2010 Dec 14;5(12):e15617. doi: 10.1371/journal.pone.0015617.
7
SmedGD 2.0: The Schmidtea mediterranea genome database.SmedGD 2.0：地中海涡虫基因组数据库。
Genesis. 2015 Aug;53(8):535-46. doi: 10.1002/dvg.22872. Epub 2015 Jul 17.
8
The freshwater planarian Schmidtea mediterranea: embryogenesis, stem cells and regeneration.淡水涡虫地中海扁形虫：胚胎发生、干细胞与再生
Curr Opin Genet Dev. 2003 Aug;13(4):438-44. doi: 10.1016/s0959-437x(03)00082-0.
9
Histone modifications and regeneration in the planarian Schmidtea mediterranea.涡虫 Schmidtea mediterranea 中的组蛋白修饰和再生。
Curr Top Dev Biol. 2014;108:71-93. doi: 10.1016/B978-0-12-391498-9.00004-8.
10
Maintenance of in the Laboratory.实验室中的维护。 （你提供的原文“Maintenance of in the Laboratory.”似乎不完整，这里是根据现有内容翻译的）
Bio Protoc. 2018 Oct 5;8(19):e3040. doi: 10.21769/BioProtoc.3040.
引用本文的文献
1
Regeneration leads to global tissue rejuvenation in aging sexual planarians.再生导致衰老的有性涡虫出现整体组织年轻化。
Nat Aging. 2025 May;5(5):780-798. doi: 10.1038/s43587-025-00847-9. Epub 2025 Apr 3.
2
Impact of host physiology and external stressors on the bacterial community of Schmidtea mediterranea.宿主生理学和外部应激源对地中海涡虫细菌群落的影响。
Sci Rep. 2025 Feb 5;15(1):4398. doi: 10.1038/s41598-025-86920-0.
3
Unveiling the Subtle Threats: The Neurobehavioral Impact of Chlorpyrifos on .揭示细微威胁：毒死蜱对……的神经行为影响
Toxics. 2024 Jul 15;12(7):512. doi: 10.3390/toxics12070512.
4
A Planarian Model System to Study Host-Pathogen Interactions.涡虫模型系统用于研究宿主-病原体相互作用。
Methods Mol Biol. 2023;2680:231-244. doi: 10.1007/978-1-0716-3275-8_14.
5
as a Model Organism to Study the Molecular Background of Human Motile Ciliopathies.作为一种研究人类运动纤毛疾病分子背景的模式生物。
Int J Mol Sci. 2023 Feb 24;24(5):4472. doi: 10.3390/ijms24054472.
6
Ca Signaling and Regeneration.钙信号转导与再生
Cold Spring Harb Perspect Biol. 2019 Nov 1;11(11):a035485. doi: 10.1101/cshperspect.a035485.
7
Towards High-Throughput Chemobehavioural Phenomics in Neuropsychiatric Drug Discovery.迈向神经精神药物发现中的高通量化学生物行为表型组学。
Mar Drugs. 2019 Jun 6;17(6):340. doi: 10.3390/md17060340.
8
Lethal and sub-lethal effects of cyproconazole on freshwater organisms: a case study with Chironomus riparius and Dugesia tigrina.三唑酮对淡水生物的致死和亚致死效应：以摇蚊和水螅为例的研究。
Environ Sci Pollut Res Int. 2018 Apr;25(12):12169-12176. doi: 10.1007/s11356-017-1180-y. Epub 2018 Feb 18.
9
Genome-Wide Analysis of Polyadenylation Events in Schmidtea mediterranea.地中海涡虫多聚腺苷酸化事件的全基因组分析
G3 (Bethesda). 2016 Oct 13;6(10):3035-3048. doi: 10.1534/g3.116.031120.
10
Planarians as a model of aging to study the interaction between stem cells and senescent cells in vivo.涡虫作为衰老模型用于研究体内干细胞与衰老细胞之间的相互作用。
Pathobiol Aging Age Relat Dis. 2015 Dec 10;5:30052. doi: 10.3402/pba.v5.30052. eCollection 2015.
本文引用的文献
1
Live Imaging of Planarian Membrane Potential Using DiBAC4(3).使用DiBAC4(3)对涡虫膜电位进行实时成像。
CSH Protoc. 2008 Oct 1;2008:pdb.prot5055. doi: 10.1101/pdb.prot5055.
2
Gene knockdown in planarians using RNA interference.利用RNA干扰技术对涡虫进行基因敲低
CSH Protoc. 2008 Oct 1;2008:pdb.prot5054. doi: 10.1101/pdb.prot5054.
3
Establishing and maintaining a colony of planarians.建立并维持涡虫种群。
CSH Protoc. 2008 Oct 1;2008:pdb.prot5053. doi: 10.1101/pdb.prot5053.
4
Planarian PTEN homologs regulate stem cells and regeneration through TOR signaling.涡虫PTEN同源物通过TOR信号传导调节干细胞和再生。
Dis Model Mech. 2008 Sep-Oct;1(2-3):131-43; discussion 141. doi: 10.1242/dmm.000117. Epub 2008 Sep 18.
5
Gene nomenclature guidelines for the planarian Schmidtea mediterranea.地中海涡虫（Schmidtea mediterranea）的基因命名指南。
Dev Dyn. 2008 Nov;237(11):3099-101. doi: 10.1002/dvdy.21623.
6
Cell turnover and adult tissue homeostasis: from humans to planarians.细胞更新与成体组织稳态：从人类到涡虫
Annu Rev Genet. 2007;41:83-105. doi: 10.1146/annurev.genet.41.110306.130244.
7
Planarians, a tale of stem cells.涡虫：一个关于干细胞的故事
Cell Mol Life Sci. 2008 Jan;65(1):16-23. doi: 10.1007/s00018-007-7426-y.
8
Regenerating the central nervous system: how easy for planarians!再生中枢神经系统：涡虫是多么容易做到啊！
Dev Genes Evol. 2007 Dec;217(11-12):733-48. doi: 10.1007/s00427-007-0188-6. Epub 2007 Nov 13.
9
SmedGD: the Schmidtea mediterranea genome database.SmedGD：地中海涡虫基因组数据库。
Nucleic Acids Res. 2008 Jan;36(Database issue):D599-606. doi: 10.1093/nar/gkm684. Epub 2007 Sep 18.
10
smedinx-11 is a planarian stem cell gap junction gene required for regeneration and homeostasis.smedinx-11是一种涡虫干细胞间隙连接基因，对再生和体内平衡至关重要。
Development. 2007 Sep;134(17):3121-31. doi: 10.1242/dev.006635. Epub 2007 Aug 1.
Zotero 插件