Department of Human Anatomy and Histology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain.
Histol Histopathol. 2011 Apr;26(4):461-70. doi: 10.14670/HH-26.461.
Studies in vitro have permitted the identification of enteric neural progenitor cells. Now the question arises as to where these progenitor cells are located in vivo. The purpose of this paper is to identify possible candidate cells by means of transmission electron microscopy (TEM). We have located three interstitial cellular types around the rat duodenum myenteric plexus. Type I cells have been identified as Interstitial Cells of Cajal (ICCs). These cells present well defined ultrastructural characteristics, including the triple connexion IC- nervous trunk- blood vessels. Type II cells show characteristics of immature cells, emphasizing the presence of a single cilium with the structure (9+0). To analyse this nanostructure, we have elaborated a reconstruction on ultrathin sections. The two previously described cellular types could be considered to be different functional states of the same cell. Type III cells present ultrastructural characteristics of fibroblast-like cells. This study suggests that Type II cells could be a source of neural progenitor cells.
体外研究已经允许鉴定出肠神经祖细胞。现在的问题是这些祖细胞在体内的位置。本文的目的是通过透射电子显微镜（TEM）来确定可能的候选细胞。我们已经在大鼠十二指肠肌间神经丛周围定位了三种间质细胞类型。I 型细胞已被鉴定为 Cajal 间质细胞（ICCs）。这些细胞具有明确的超微结构特征，包括三联连接 ICC-神经干-血管。II 型细胞表现出不成熟细胞的特征，强调存在具有结构（9+0）的单个纤毛。为了分析这种纳米结构，我们在超薄切片上进行了重建。这两种先前描述的细胞类型可以被认为是同一细胞的不同功能状态。III 型细胞具有成纤维样细胞的超微结构特征。本研究表明，II 型细胞可能是神经祖细胞的来源。
