[通脉颗粒的高效液相色谱-二极管阵列检测-质谱指纹图谱]
[Fingerprints of Tongmai Keli by HPLC-DAD-MS].
作者信息
Li Bei-Jia, Xiang Cheng, Yang Xiu-Wei, Wu Li-Jun, Guo De-An, Ye Min
机构信息
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
出版信息
Yao Xue Xue Bao. 2010 Nov;45(11):1410-4.
In order to clarify the chemical composition of Tongmai Keli, a HPLC fingerprint was established, and the 22 peaks were characterized by LC-DAD-MS. The herbal sources of these peaks were assigned. The results implied that genistin 8-C-glucoside, puerarin, daidzein 8-C-apiosyl (1, 6)-O-glucoside, daidzin, and salvianolic acid B were the main constituents of in Tongmai Keli. Ten batches of Tongmai Keli produced by different pharmaceutical companies were analyzed. Although different batches contained similar compounds, the contents of major compounds were significantly different. The method established in this study could be used for the quality control of Tongmai Keli.
为阐明通脉颗粒的化学成分，建立了高效液相色谱指纹图谱，并通过液相色谱-二极管阵列检测器-质谱联用技术对22个峰进行了表征。确定了这些峰的药材来源。结果表明，染料木苷8-C-葡萄糖苷、葛根素、大豆苷元8-C-芹菜糖基（1,6）-O-葡萄糖苷、大豆苷和丹酚酸B是通脉颗粒的主要成分。对不同制药公司生产的十批通脉颗粒进行了分析。虽然不同批次含有相似的化合物，但主要化合物的含量存在显著差异。本研究建立的方法可用于通脉颗粒的质量控制。
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