Curriculum in Toxicology, University of North Carolina at Chapel Hill, NC, USA.
Chem Res Toxicol. 2011 Apr 18;24(4):478-80. doi: 10.1021/tx200060c. Epub 2011 Mar 11.
Growing evidence suggest that the methylated trivalent metabolites of inorganic arsenic (iAs), methylarsonite (MAs(III)) and dimethylarsinite (DMAs(III)), contribute to adverse effects of iAs exposure. However, the lack of suitable methods has hindered the quantitative analysis of MAs(III) and DMAs(III) in complex biological matrices. Here, we show that hydride generation-cryotrapping-atomic absorption spectrometry can quantify both MAs(III) and DMAs(III) in livers of mice exposed to iAs. No sample extraction is required, thus limiting MAs(III) or DMAs(III) oxidation prior to analysis. The limits of detection are below 6 ng As/g of tissue, making this method suitable even for studies examining low exposures to iAs.
越来越多的证据表明，无机砷（iAs）的三价甲基代谢物，即甲基胂酸（MAs(III)）和二甲基砷酸（DMAs(III)），会导致 iAs 暴露的不良影响。然而，缺乏合适的方法阻碍了复杂生物基质中 MAs(III)和 DMAs(III)的定量分析。在这里，我们展示了氢化物发生-冷阱-原子吸收光谱法可以定量分析 iAs 暴露的小鼠肝脏中的 MAs(III)和 DMAs(III)。不需要样品提取，从而限制了分析前 MAs(III)或 DMAs(III)的氧化。检测限低于 6 ng As/g 组织，即使是研究 iAs 低暴露的研究也非常适用。
