Department of Nutrition, University of California, Davis, California 95616, United States.
J Proteome Res. 2011 Apr 1;10(4):1746-54. doi: 10.1021/pr101028k. Epub 2011 Mar 1.
Human milk is a rich source of bioactive proteins that support the early growth and development of the newborn. Although the major components of the protein fraction in human milk have been studied, the expression and relative abundance of minor components have received limited attention. We examined the expression of low-abundance proteins in the whey fraction of human milk and their dynamic changes over a twelve-month lactation period. The low-abundance proteins were enriched by ProteoMiner beads, and protein identification was performed by liquid chromatography tandem mass spectrometry. One hundred and fifteen proteins were identified, thirty-eight of which have not been previously reported in human colostrum or milk. We also for the first time described differences in protein patterns among the low-abundance proteins during lactation. These results enhance our knowledge about the complexity of the human milk proteome, which constitutes part of the advantages to the breast-fed infant.
人乳是生物活性蛋白质的丰富来源，这些蛋白质支持新生儿的早期生长和发育。虽然已经研究了人乳蛋白质部分的主要成分，但对次要成分的表达和相对丰度的关注有限。我们研究了人乳乳清部分中低丰度蛋白质的表达及其在 12 个月哺乳期内的动态变化。使用 ProteoMiner 珠将低丰度蛋白质进行富集，并通过液相色谱串联质谱法进行蛋白质鉴定。鉴定出 115 种蛋白质，其中 38 种在人初乳或乳中以前没有报道过。我们还首次描述了哺乳期低丰度蛋白质之间蛋白质图谱的差异。这些结果增强了我们对人乳蛋白质组复杂性的认识，这是人乳喂养婴儿的优势之一。
