Institute of Photonic Technology e.V., Albert-Einstein-Strasse 9, 07745 Jena, Germany.
J Biomed Opt. 2011 Feb;16(2):021113. doi: 10.1117/1.3533268.
Contemporary brain tumor research focuses on two challenges: First, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. Second, for prognostication the tumor has to be removed as completely as possible. Nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. However, it is neither applicable in vivo, nor does it allow for precise tumor typing in those cases when only nonrepresentative specimens are procured. Infrared and Raman spectroscopy allow for very precise cancer analysis due to their molecular specificity, while nonlinear microscopy is a suitable tool for rapid imaging of large tissue sections. Here, unstained samples from the brain of a domestic pig have been investigated by a multimodal nonlinear imaging approach combining coherent anti-Stokes Raman scattering, second harmonic generation, and two photon excited fluorescence microscopy. Furthermore, a brain tumor specimen was additionally analyzed by linear Raman and Fourier transform infrared imaging for a detailed assessment of the tissue types that is required for classification and to validate the multimodal imaging approach. Hence label-free vibrational microspectroscopic imaging is a promising tool for fast and precise in vivo diagnostics of brain tumors.
首先，通过分析切除的组织对肿瘤进行分类和分级，对于选择治疗方法至关重要。其次，为了进行预后评估，必须尽可能完全切除肿瘤。目前，使用苏木精-伊红染色对切除的组织进行组织病理学检查是手术病理标本明确诊断的金标准。然而，它既不适用于体内，也不能在仅获得非代表性标本的情况下进行精确的肿瘤分类。由于具有分子特异性，红外和拉曼光谱可非常精确地进行癌症分析，而非线性显微镜则是快速成像大组织切片的合适工具。在这里，我们通过结合相干反斯托克斯拉曼散射、二次谐波产生和双光子激发荧光显微镜的多模态非线性成像方法，对来自家猪大脑的未经染色的样本进行了研究。此外，还通过线性拉曼和傅里叶变换红外成像对脑肿瘤标本进行了分析，以详细评估组织类型，这是分类所必需的，并验证了多模态成像方法。因此，无标记振动显微光谱成像技术是一种快速、精确的脑肿瘤体内诊断的有前途的工具。
