Prabhu N, Innocent Joseph Pushpa, Chinnaswamy P, Natarajaseenivasan K, Sarayu Lakshmi
Postgraduate and Research Department of Microbiology, Dr. NGP Arts and Science College, Coimbatore-641 035, India.
Indian J Pharm Sci. 2008 Nov;70(6):788-91. doi: 10.4103/0250-474X.49124.
Leptospirosis is now acknowledged as the most widespread zoonoses in the world. Hundreds of cases occur in India every year accounting for considerable morbidity and sizable mortality. Several studies have delineated the epidemiology, pathology and variable clinical features of this condition. The present study comprises the importance and utilization of traditional based medicines to overcome the adverse reaction by conventional drugs and standardize the technology. The antileptospiral activity of Eclipta alba L. was well studied by both tube dilution and micro dilution techniques and the result showed better inhibitory action against various serogroups of Leptospira interrogans. L. australis, L. autumnalis and L. grippotyphosa are inhibited by both water and ethanol extract by tube dilution technique. The MIC level observed are 50 μg and 100 μg respectively. Similarly acetone extract, Icterohaemorrhagiae was responded to 200 μg/ml as MIC whereas in petroleum ether extract, no inhibition was observed. In the case of micro dilution technique, the entire inhibition rates are supported to the tube dilution technique. It showed that the micro dilution technique is the best method where we obtained the results within 30 minutes; at the same time tube dilution technique takes minimum of 7 days to provide the result.
钩端螺旋体病现已被公认为世界上分布最广的人畜共患病。印度每年有数百例病例，导致相当高的发病率和可观的死亡率。多项研究已经阐明了这种疾病的流行病学、病理学和多样的临床特征。本研究包括传统药物的重要性及其在克服传统药物不良反应和规范技术方面的应用。通过试管稀释法和微量稀释法对旱莲草的抗钩端螺旋体活性进行了深入研究，结果表明其对问号钩端螺旋体的各种血清群具有更好的抑制作用。通过试管稀释法，澳洲钩端螺旋体、秋季钩端螺旋体和黄疸出血型钩端螺旋体均受到水提取物和乙醇提取物的抑制。观察到的最低抑菌浓度水平分别为50微克和100微克。同样，丙酮提取物对出血黄疸型钩端螺旋体的最低抑菌浓度为200微克/毫升，而石油醚提取物未观察到抑制作用。在微量稀释法中，所有的抑制率都与试管稀释法一致。结果表明，微量稀释法是最佳方法，能在30分钟内得出结果；而试管稀释法至少需要7天才能得出结果。
