Department of Clinical Analysis and Toxicology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Mycopathologia. 2011 Aug;172(2):125-30. doi: 10.1007/s11046-011-9407-9. Epub 2011 Mar 3.
Currently, there has been an increased frequency of fungal infections. Candida albicans and other Candida spp. have been proven to be major causes for urinary tract infection. Increased resistance to antifungals indicates the need to develop strategies in order to prevent the spread of resistance. Chromogenic medium have been proven to be useful in the detection of yeasts in clinical specimens containing mixed cultures of Candida. The aim of this study was to compare the results of antifungal susceptibility testing with fluconazole and amphotericin B on strains of Candida spp. isolated from urine, conducted on a Mueller-Hinton Agar with Glucose and Methylene Blue (MHAGMB) medium and on a Hicrome Candida® Agar with 2% Glucose (HCAG) medium. We used 40 samples of Candida spp. isolated from urine samples from inpatients and outpatients. The results showed that both media presented high rates of agreement, above 94%. The use of the HCAG medium decreases the release time of the results by 24-48 h, which may be decisive for initiating the correct drug treatment.
目前，真菌感染的频率有所增加。白色念珠菌和其他念珠菌已被证明是尿路感染的主要原因。抗真菌药物的耐药性增加表明需要制定策略以防止耐药性的传播。显色培养基已被证明可用于检测含有念珠菌混合培养物的临床标本中的酵母菌。本研究旨在比较氟康唑和两性霉素 B 对从尿液中分离出的念珠菌属菌株的抗真菌药敏试验结果，该试验在含有葡萄糖和亚甲蓝的 Mueller-Hinton 琼脂（MHAGMB）培养基和含有 2%葡萄糖的 Hicrome Candida®琼脂（HCAG）培养基上进行。我们使用了 40 份从住院和门诊患者尿液样本中分离出的念珠菌属样本。结果表明，两种培养基的符合率均高于 94%。使用 HCAG 培养基可将结果的释放时间缩短 24-48 小时，这对于启动正确的药物治疗可能是决定性的。
