Department of Chemical and Materials Engineering, School of Engineering, University of Dayton, 300 College Park, Dayton, Ohio 45469, United States.
ACS Nano. 2011 Mar 22;5(3):2376-84. doi: 10.1021/nn200279k. Epub 2011 Mar 3.
Because of their unique photoluminescence and magnetic properties, nanodiamonds (NDs) are promising for biomedical imaging and therapeutical applications. However, these biomedical applications will hardly be realized unless the potential hazards of NDs to humans and other biological systems are ascertained. Previous studies performed in our group and others have demonstrated the excellent biocompatibility of NDs in a variety of cell lines without noticeable cytotoxicity. In the present paper, we report the first genotoxicity study on NDs. Our results showed that incubation of embryonic stem cells with NDs led to slightly increased expression of DNA repair proteins, such as p53 and MOGG-1. Oxidized nanodiamonds (O-NDs) were demonstrated to cause more DNA damage than the pristine/raw NDs (R-NDs), showing the surface chemistry specific genotoxicity. However, the DNA damages caused by either the O-NDs or the R-NDs are much less severe than those caused by multiwalled carbon nanotubes (MWNTs) observed in our previous study. These findings should have important implications for future applications of NDs in biological applications.
由于纳米金刚石（NDs）具有独特的光致发光和磁性，因此在生物医学成像和治疗应用方面具有广阔的前景。然而，除非确定 NDs 对人类和其他生物系统的潜在危害，否则这些生物医学应用几乎不可能实现。我们小组和其他小组的先前研究已经证明，NDs 在各种细胞系中具有优异的生物相容性，没有明显的细胞毒性。在本论文中，我们报告了对 NDs 的首次遗传毒性研究。我们的结果表明，NDs 孵育胚胎干细胞导致 DNA 修复蛋白（如 p53 和 MOGG-1）的表达略有增加。已证明氧化纳米金刚石（O-NDs）比原始/未处理的纳米金刚石（R-NDs）引起更多的 DNA 损伤，表明表面化学具有特定的遗传毒性。然而，与我们之前研究中观察到的多壁碳纳米管（MWNTs）相比，O-NDs 或 R-NDs 引起的 DNA 损伤要轻得多。这些发现对于 NDs 在生物应用中的未来应用具有重要意义。
