Australian Institute for Bioengineering and Nanotechnology and the School of Chemical Engineering, The University of Queensland, St. Lucia, Queensland, Australia.
Acta Biomater. 2011 Jun;7(6):2544-57. doi: 10.1016/j.actbio.2011.02.036. Epub 2011 Mar 1.
This study investigates the use of patterned collectors to increase the pore size of electrospun scaffolds for enhanced cell infiltration. The morphology of the patterned scaffolds was investigated by scanning electron microscopy, which showed that the collector pattern was accurately mimicked by the electrospun fibres. We observed an enlargement in the pore size and in the pore size distribution compared with conventional electrospinning. Mechanical testing revealed that the mechanical properties could be tailored, to some extent, according to the patterning and that the patterned scaffolds were softer than standard electrospun scaffolds. When NIH 3T3 fibroblasts were seeded onto patterned collectors improved cell infiltration was observed. Cells were able to penetrate up to 250 μm into the scaffolds, compared with 30 μm for the standard scaffolds. This increase in the depth of infiltration occurred as early as 24 h post-seeding and remained constant over 7 days.
本研究探讨了使用图案收集器来增加电纺支架的孔径，以增强细胞渗透。通过扫描电子显微镜研究了图案支架的形态，结果表明电纺纤维准确地模拟了收集器的图案。我们观察到与传统电纺相比，孔径和孔径分布都有所增大。力学测试表明，在一定程度上可以根据图案来调整力学性能，而且图案支架比标准电纺支架更柔软。当 NIH 3T3 成纤维细胞接种到图案收集器上时，观察到细胞渗透得到改善。与标准支架的 30μm 相比，细胞能够渗透到支架中 250μm 的深度。这种渗透深度的增加早在接种后 24 小时就发生了，并在 7 天内保持不变。
