Peking University Third Hospital, No, 49, North Huayuan Road, Haidian District, Beijing 100191, PR China.
Reprod Biol Endocrinol. 2011 Mar 6;9:29. doi: 10.1186/1477-7827-9-29.
To determine the effect of higher progesterone (P) level on endometrial receptivity.
This was a prospective analysis conducted in the Reproductive Medical Center of Peking University Third Hospital. All patients received IVF treatment and canceled embryo transfer in the same cycle and were divided into group 1 (normal P; 7 patients) and group 2 (elevated P; 12 patients). Endometrial biopsies were performed 6 days after oocyte retrieval. The global miRNA and mRNA gene expressions in endometrial biopsies were investigated with a V4.0 miRNA probe and 22 K Human Genome Array. Fold ratios were derived to compare gene regulation between the groups. Spp1 and Ang gene expression was selected to verify the array results by RT-PCR and the protein expression of osteopontin and VEGF was determined using an immunohistochemical method.
There were 4 miRNA (all down-regulated) and 22 mRNA (13 up-regulated and 9 down-regulated) exhibiting differential expression between the groups on the microRNA and microarray chips. miRNA-451, Spp1, and Ang expression in RT-PCR verified the array results. Osteopontin and VEGF were also shown to have positive expression in the endometrium.
Data from microRNA and microarray analysis suggests dissimilar endometrial receptivity in patients with high P levels on the day of hCG, and elevated osteopontin and decreased VEGF had poor pregnancy rates.
为了确定孕激素（P）水平升高对子宫内膜容受性的影响。
这是一项在北京大学生殖医学中心进行的前瞻性分析。所有患者均接受体外受精治疗，并在同一周期取消胚胎移植，分为第 1 组（正常 P；7 例）和第 2 组（升高 P；12 例）。取卵后 6 天进行子宫内膜活检。使用 V4.0 miRNA 探针和 22K 人类基因组阵列检测子宫内膜活检中的全局 miRNA 和 mRNA 基因表达。采用倍数比比较两组之间的基因调控。选择 Spp1 和 Ang 基因表达通过 RT-PCR 验证阵列结果，并通过免疫组织化学方法确定骨桥蛋白和 VEGF 的蛋白表达。
在 miRNA 和微阵列芯片上，两组之间有 4 个 miRNA（均下调）和 22 个 mRNA（13 个上调和 9 个下调）表现出差异表达。RT-PCR 验证了 miRNA-451、Spp1 和 Ang 的表达结果。骨桥蛋白和 VEGF 在子宫内膜中也表现出阳性表达。
miRNA 和微阵列分析的数据表明，在 hCG 日孕激素水平升高的患者中，子宫内膜容受性不同，骨桥蛋白升高和 VEGF 降低的妊娠率较差。
