Combined effects of titanium dioxide and humic acid on the bioaccumulation of cadmium in Zebrafish.

The combined effects of titanium dioxide (TiO2) nanoparticles and humic acid (HA) on the bioaccumulation of cadmium (Cd) in Zebrafish were investigated. Experimental data on the equilibrium Cd bioaccumulation suggest that only the dissolved Cd effectively contributed to Cd bioaccumulation in HA solutions whereas both the dissolved and TiO2 associated Cd were accumulated in TiO2 or the mixture of HA and TiO2 solutions, due likely to the additional intestine uptake of the TiO2-bound Cd. The equilibrium Cd bioaccumulation in the mixed system was comparable to that in the corresponding HA solutions, and significantly lower than that in the corresponding TiO2 solutions (n=3, p<0.05). The presence of either HA or TiO2 (5-20 mg L(-1)) in water slightly increased the uptake rate constants of Cd bioaccumulation whereas combining HA and TiO2 reduced the uptake rate constants.