De Fusco R, Monarca S, Biscardi D, Pasquini R, Fatigoni C
Department of Experimental Pharmacology, University of Naples, Italy.
Sci Total Environ. 1990 Jan;90:241-8. doi: 10.1016/0048-9697(90)90196-2.
Polyethyleneterephthalate (PET) was tested as a source of mutagen contamination from bottles used for beverage packaging. PET bottles were filled with mineral water and stored in daylight and in the dark for different periods of time. The water samples were concentrated and the concentrates (non-volatile compounds) tested for mutagenicity with the Ames test (static tests). Total organic carbon (TOC) leaching was determined concurrently. Leaching of mutagens was also studied using dynamic tests; shaking distilled water in PET bottles. New methods were also used to test the leaching potential of both volatile and non-volatile compounds: directly testing the mutagenicity in unconcentrated water stored in PET bottles and growing Salmonella strains directly in the plastic bottles. The results were positive only for the static test, which identified leaching of mutagens after 1 month of storage in PET bottles. This activity was higher after storage in daylight.
对聚对苯二甲酸乙二酯（PET）作为饮料包装瓶中诱变污染物来源进行了测试。PET瓶装满矿泉水，分别在日光和黑暗环境下储存不同时长。对水样进行浓缩，并用艾姆斯试验（静态试验）检测浓缩物（非挥发性化合物）的致突变性。同时测定总有机碳（TOC）的浸出量。还通过动态试验研究诱变剂的浸出情况，即将蒸馏水在PET瓶中振荡。还采用新方法测试挥发性和非挥发性化合物的浸出潜力：直接检测储存在PET瓶中未浓缩水中的致突变性，以及直接在塑料瓶中培养沙门氏菌菌株。仅静态试验结果呈阳性，该试验确定PET瓶储存1个月后有诱变剂浸出。在日光下储存后的这种活性更高。
