三萜皂苷影响 P-糖蛋白的功能并降低自由生活阶段的贝氏肉孢子虫的存活率。

Triterpenoid saponins affect the function of P-glycoprotein and reduce the survival of the free-living stages of Heligmosomoides bakeri.

机构信息

Institute of Zoology, University of Warsaw, Warsaw, Poland.

出版信息

Vet Parasitol. 2011 Jun 30;179(1-3):144-51. doi: 10.1016/j.vetpar.2011.01.053. Epub 2011 Feb 2.

DOI:10.1016/j.vetpar.2011.01.053

Abstract

We studied the effect of triterpenoid saponins on the development of free-living stages of Heligmosomoides bakeri, a parasitic nematode of the mouse intestine. We evaluated the effectiveness of oleane-type glucuronides (GlcUAOA) isolated from Calendula officinalis and Beta vulgaris. The rhodamine 123 retention assay was used to detect dysfunctions of the major membrane transporter for xenobiotics, P-glycoprotein (Pgp). Both C. officinals and B. vulgaris GlcUAOA affect the development of the free living stages and function of Pgp in H. bakeri. The GlcUAOA inhibits egg hatching and moulting of larvae and also changes their morphology. These saponin fractions reversed the toxic effect of thiabendazole on the nematode; the function of Pgp was also inhibited.

摘要

我们研究了三萜皂苷对自由生活阶段的贝氏蛔虫（一种寄生在老鼠肠道中的线虫）发育的影响。我们评估了从金盏花和糖萝卜中分离出的齐墩果烷型葡萄糖醛酸苷（GlcUAOA）的有效性。使用罗丹明 123 保留试验来检测异生物质主要膜转运蛋白 P-糖蛋白（Pgp）的功能障碍。金盏花和糖萝卜的 GlcUAOA 均影响自由生活阶段贝氏蛔虫的发育和 Pgp 的功能。GlcUAOA 抑制卵的孵化和幼虫的蜕皮，并改变其形态。这些皂苷部分逆转了噻苯达唑对线虫的毒性作用；Pgp 的功能也受到了抑制。