Laboratoire Sciences Chimiques de Rennes, UMR CNRS 6226, Ecole Nationale Supérieure de Chimie de Rennes, Avenue du Général Leclerc, CS 50837, 35708 Rennes, Cedex 7, France.
Talanta. 2011 Apr 15;84(2):513-8. doi: 10.1016/j.talanta.2011.01.034. Epub 2011 Jan 22.
In this article, the radical-scavenging capacity of phenol fractions extracted from the brown seaweed Ascophyllum nodosum was assessed using in parallel colorimetric methods (ABTS and DPPH) and electrochemistry (cyclic voltammetry). Results obtained by the three methods correlated in the case of global fractions, whereas only ABTS and DPPH correlated when activities were expressed on a phenol basis. The successive fractions separated by both their average molecular size and their polarity exhibited activities largely dependent on their phenol content, suggesting that phlorotannins are the main anti-oxidant molecules in hydro-alcoholic extracts of A. nodosum. In addition, phenol fractions of relative low molecular weight were clearly more active than others. This work opens new opportunities to better evaluate the radical-scavenging potential of phenol pools in algae using both bi-parametric fractionating and electrochemistry.
本文采用平行比色法（ABTS 和 DPPH）和电化学法（循环伏安法）评估了来自褐藻泡叶藻的酚类提取物的自由基清除能力。三种方法的结果在整体分数的情况下相关，而当以酚类为基础表达活性时，只有 ABTS 和 DPPH 相关。通过平均分子量和极性对两种方法分离的连续分数显示，其活性主要取决于其酚类含量，表明在泡叶藻的水醇提取物中，岩藻多酚是主要的抗氧化分子。此外，相对低分子量的酚类分数明显比其他分数更活跃。这项工作为使用双参数分级和电化学更好地评估藻类中酚类库的自由基清除潜力提供了新的机会。
