Laboratoire de Biomathématiques, Biochimie, Biophysique et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria.
Laboratoire de Biochimie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, Algeria.
Molecules. 2023 Jan 6;28(2):571. doi: 10.3390/molecules28020571.
The use of seeds in the food, pharmaceutical, and cosmetic fields is common, since the iniquity and the virtues of these plants are directly related to their characteristic phytochemical composition. This investigation focused on the comparative study of the botanical aspect, phenolic profile, and in vitro and in vivo biological activities of L. (NS) and L. (ND) seeds. The macro- and micro-morphological properties of these seeds were studied, and the key dissimilarities between them were clearly illustrated. The phytochemical contents and phenolic profiles were determined, and the in vitro antioxidant activity was assessed using four methods. The in vivo antioxidant and biochemical parameters of the blood of supplemented mice were determined. The results of the macro- and micro-structure analysis revealed differences between the two plants. Here, ND is characterized by higher phytochemical contents and the best antioxidant activities. The HPLC analysis indicated the presence of nine compounds, namely seven phenolic acids, particularly hydroxybenzoic and caffeic acids, and two flavonoids. The administration of ND extract to mice for 21 days at a concentration of 500 mg/kg allowed a substantial amelioration of plasma antioxidant properties. In addition, the extracts ameliorate blood parameters (cholesterol, triglycerides, glycemia, and urea). Furthermore, the antimicrobial activity of extracts demonstrated their effects on and . seeds, in particular ND, expressed considerable in vitro antioxidant properties and demonstrated significant amelioration of mice blood properties. Consequently, these species can serve as a valuable source of compounds with various applications.
种子在食品、制药和化妆品领域的应用很常见，因为这些植物的邪恶与美德与其特有的植物化学成分直接相关。本研究重点比较了 L.（NS）和 L.（ND）种子的植物学方面、酚类成分、体外和体内生物活性。对这些种子的宏观和微观形态特性进行了研究，并清楚地说明了它们之间的关键差异。测定了这些种子的植物化学含量和酚类成分，并采用四种方法评估了体外抗氧化活性。还测定了补充了这些种子提取物的小鼠血液中的抗氧化和生化参数。宏观和微观结构分析的结果显示了这两种植物之间的差异。在这里，ND 的植物化学含量更高，具有最佳的抗氧化活性。HPLC 分析表明存在九种化合物，即七种酚酸，特别是羟基苯甲酸和咖啡酸，以及两种类黄酮。在 500mg/kg 浓度下，将 ND 提取物给小鼠喂食 21 天，可显著改善血浆抗氧化性能。此外，提取物可改善血液参数（胆固醇、甘油三酯、血糖和尿素）。此外，提取物的抗菌活性表明它们对 和 的作用。L. 种子，特别是 ND，表现出相当强的体外抗氧化性能，并显著改善了小鼠的血液特性。因此，这些物种可以作为具有多种应用的化合物的有价值来源。
