Laboratory of Chemical, Galenic and Pharmacological Development of Drugs, Faculty of Pharmacy of Monastir, University of Monastir, 5000, Monastir, Tunisia.
Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity, Medicinal Chemistry and Natural Products Team, Faculty of Sciences of Monastir, University of Monastir, 5019, Monastir, Tunisia.
Mar Biotechnol (NY). 2018 Feb;20(1):60-74. doi: 10.1007/s10126-017-9787-z. Epub 2018 Jan 18.
Phlorotannins, phenolic compounds produced exclusively by seaweeds, have been reported to possess various pharmacological properties. However, there have been few works on these compounds from Mediterranean seaweeds. In this study, we investigated the phytochemical analysis and pharmacological potential of phlorotannin-rich fractions from three brown seaweeds collected along the Tunisia coast: Cystoseira sedoides (PHT-SED), Cladostephus spongeosis (PHT-CLAD), and Padina pavonica (PHT-PAD). Phytochemical determinations showed considerable differences in total phenolic content (TPC) and phlorotannin content (PHT). The highest TPC level (26.45 mg PGE/g dry material (Dm)) and PHT level (873.14 μg PGE/g Dm) were observed in C. sedoides. The antioxidant properties of these three fractions assessed by three different methods indicated that C. sedoides displayed the highest total antioxidant activity among the three species (71.30 mg GAE/g Dm), as well as the free radical scavenging activity with the lowest IC value in both DPPH (27.7 μg/mL) and ABTS (19.1 μg/mL) assays. Furthermore, the pharmacological screening of the anti-inflammatory potential of these fractions using in vivo models, in comparison to reference drugs, established a remarkable activity of PHT-SED at the dose of 100 mg/kg; the inhibition percentages of ear edema in mice model and paw edema in rats model were of 82.55 and 81.08%, respectively. The content of malondialdehyde (MDA) in liver tissues has been quantified, and PHT-SED was found to remarkably increase the lipid peroxidation in rat liver tissues. In addition, in two pain mice models, PHT-SED displayed a profound antinociceptive activity at 100 mg/kg and has proved a better analgesic activity when used in combination with the opioid drug, tramadol.
岩藻黄质是一种仅由海藻产生的酚类化合物，据报道具有多种药理特性。然而，关于地中海海藻中的这些化合物的研究很少。在这项研究中，我们研究了三种沿突尼斯海岸采集的褐藻中的岩藻黄质丰富部分的植物化学分析和药理潜力：Cystoseira sedoides（PHT-SED）、Cladostephus spongeosis（PHT-CLAD）和Padina pavonica（PHT-PAD）。植物化学测定显示总酚含量（TPC）和岩藻黄质含量（PHT）有相当大的差异。C. sedoides 中观察到最高的 TPC 水平（26.45 mg PGE/g 干物质（Dm））和 PHT 水平（873.14μg PGE/g Dm）。三种方法评估的这三种馏分的抗氧化性能表明，C. sedoides 在三种物种中表现出最高的总抗氧化活性（71.30 mg GAE/g Dm），以及在 DPPH（27.7μg/mL）和 ABTS（19.1μg/mL）测定中具有最低 IC 值的自由基清除活性。此外，与参比药物相比，使用体内模型对这些馏分的抗炎潜力进行药理筛选，确立了 PHT-SED 在 100mg/kg 剂量下的显著活性；在小鼠耳水肿模型和大鼠足肿胀模型中，抑制率分别为 82.55%和 81.08%。还定量了肝组织中丙二醛（MDA）的含量，发现 PHT-SED 可显著增加大鼠肝组织中的脂质过氧化。此外，在两种疼痛小鼠模型中，PHT-SED 在 100mg/kg 时表现出明显的镇痛活性，并在与阿片类药物曲马多联合使用时表现出更好的镇痛活性。
