豇豆花叶病毒纳米颗粒靶向癌细胞表面的波形蛋白。
Cowpea mosaic virus nanoparticles target surface vimentin on cancer cells.
机构信息
Department for Cell Biology, Center for Integrative Molecular Biosciences, The Scripps Research Institute, La Jolla, CA, USA.
出版信息
Nanomedicine (Lond). 2011 Feb;6(2):351-64. doi: 10.2217/nnm.10.136.
Abstract
AIMS
Vimentin, a type III intermediate filament, is upregulated during epithelial-mesenchymal transition and tumor progression. Vimentin is surface-expressed on cells involved in inflammation; the function remains unknown. We investigated the expression of surface vimentin on cancer cells and evaluated targeting nanoparticles to tumors exploiting vimentin.
MATERIALS & METHODS: Cowpea mosaic virus nanoparticles that interact with surface vimentin were used as probes. Tumor homing was tested using the chick chorioallantoic membrane model with human tumor xenografts.
RESULTS & DISCUSSION: Surface vimentin levels varied during cell cycle and among the cell lines tested. Surface vimentin expression correlated with cowpea mosaic virus uptake, underscoring the utility of cowpea mosaic virus to detect invasive cancer cells. Targeting to tumor xenografts was observed; homing was based on the enhanced permeability and retention effect. Our data provide novel insights into the role of surface vimentin in cancer and targeting nanoparticles in vivo.
摘要
目的
波形蛋白是 III 型中间丝，在上皮-间充质转化和肿瘤进展过程中上调。波形蛋白在参与炎症的细胞表面表达，其功能尚不清楚。本研究旨在研究癌细胞表面波形蛋白的表达，并评估利用波形蛋白靶向肿瘤的纳米颗粒。
材料与方法
使用与表面波形蛋白相互作用的豇豆花叶病毒纳米颗粒作为探针。用人肿瘤异种移植物在鸡胚绒毛尿囊膜模型中测试肿瘤归巢。
结果与讨论
细胞周期和测试的细胞系中表面波形蛋白的水平不同。表面波形蛋白的表达与豇豆花叶病毒的摄取相关，这突出了豇豆花叶病毒在检测侵袭性癌细胞中的应用。观察到对肿瘤异种移植物的靶向作用；归巢基于增强的通透性和保留效应。我们的数据为表面波形蛋白在癌症中的作用以及体内靶向纳米颗粒提供了新的见解。
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