Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, United States.
Mol Pharm. 2013 Jan 7;10(1):33-42. doi: 10.1021/mp300240m. Epub 2012 Jul 9.
Nanomaterials with elongated architectures have been shown to possess differential tumor homing properties compared to their spherical counterparts. Here, we investigate whether this phenomenon is mirrored by plant viral nanoparticles that are filamentous (Potato virus X) or spherical (Cowpea mosaic virus). Our studies demonstrate that Potato virus X (PVX) and Cowpea mosaic virus (CPMV) show distinct biodistribution profiles and differ in their tumor homing and penetration efficiency. Analogous to what is seen with inorganic nanomaterials, PVX shows enhanced tumor homing and tissue penetration. Human tumor xenografts exhibit higher uptake of PEGylated filamentous PVX compared to CPMV, particularly in the core of the tumor. This is supported by immunohistochemical analysis of the tumor sections, which indicates greater penetration and accumulation of PVX within the tumor tissues. The enhanced tumor homing and retention properties of PVX along with its higher payload carrying capacity make it a potentially superior platform for applications in cancer drug delivery and imaging applications.
具有细长结构的纳米材料被证明具有与球形纳米材料不同的肿瘤归巢特性。在这里，我们研究了植物病毒纳米颗粒是否具有同样的现象，这些植物病毒纳米颗粒是丝状的（马铃薯 X 病毒）或球形的（豇豆花叶病毒）。我们的研究表明，马铃薯 X 病毒（PVX）和豇豆花叶病毒（CPMV）表现出不同的生物分布特征，在肿瘤归巢和穿透效率方面也有所不同。类似于无机纳米材料，PVX 显示出增强的肿瘤归巢和组织穿透能力。人肿瘤异种移植物显示出对聚乙二醇化丝状 PVX 的摄取高于 CPMV，尤其是在肿瘤的核心部位。肿瘤切片的免疫组织化学分析也支持了这一点，这表明 PVX 在肿瘤组织内的穿透和积累更多。PVX 具有增强的肿瘤归巢和保留特性以及更高的载药能力，使其成为癌症药物输送和成像应用的潜在优越平台。
