Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China.
Chin J Integr Med. 2011 Feb;17(2):116-25. doi: 10.1007/s11655-011-0639-9. Epub 2011 Mar 9.
To study the protective effect of the Mixture of Shengmai Powder and Danshen Decoction (, abbreviated as the Mixture) in the rat model with type 2 diabetic cardiomyopathy in the rat model with type 2 diabetic cardiomyopathy, abbreviated as the Mixture) in the rat model with type 2 diabetic cardiomyopathy (DCM).
Forty-two SD rats with DCM model, established by the combination of insulin resistance by a high-fat diet with the damage of pancreatic islet β cells by intraperitoneal injection of high dose streptozotocin (50 mg/kg) once, were evaluated in the damage of the myocardium by electrocardiogram at the end of 12 weeks of grouping and intervention administration; the extent of damage in the myocardial subcellular structure was observed by electron microscopy; the content of myocardial collagen in the left cardiac ventricle was quantified by Masson staining test; the myocardial cell apoptosis was determined by TUNEL; the changes in the mRNA expression levels of thrombospodin-1 (TSP-1) and tribbles homolog 3 (TRB-3) by real-time quantitative PCR, the expression levels of myocardial TSP-1, tumor growth factorβ1 (TGF-β1), TRB-3, and chymase were detected by immunohistochemistry, and the changes in the expression levels of myocardial TSP-1, active-TGF-β1 (A-TGF-β1) and latent-TGF-β1 (L-TGF-β1) protein were tested by Western blotting.
Compared with the control group, the myocardial tissue was less damaged, and the extent of damage in the myocardial subcellular structure was less; the collagen fiber content and the cell apoptosis were reduced; the expression levels of TSP-1mRNA and TRB-3 mRNA, the expression levels of myocardial TSP-1, TGF-β1, TRB-3, and chymase, as well as the average expression levels of the myocardial TSP-1, A-TGFβ1, and L-TGF-β1 protein were decreased in the Mixture group.
The Mixture of Shengmai Powder and Danshen Decoction could inhibit the process of myocardial fibrosis in the rat myocardium of DCM through multiple pathways and significantly delay the genesis and progress of DCM in hyperglycemic rats.
研究生脉散合丹参饮（简称合剂）对 2 型糖尿病心肌病（DCM）大鼠模型心肌的保护作用。
42 只 SD 大鼠通过高脂饮食联合高剂量链脲佐菌素（50mg/kg）腹腔注射建立 DCM 模型，在分组和干预给药结束后 12 周通过心电图评估心肌损伤情况；电镜观察心肌亚细胞结构损伤情况；Masson 染色检测左心室心肌胶原含量；TUNEL 检测心肌细胞凋亡；实时定量 PCR 检测血栓调节蛋白-1（TSP-1）和 tribbles 同源蛋白 3（TRB-3）mRNA 表达水平，免疫组化检测心肌 TSP-1、转化生长因子β1（TGF-β1）、TRB-3、糜酶表达水平，Western blot 检测心肌 TSP-1、活性转化生长因子β1（A-TGF-β1）和潜伏转化生长因子β1（L-TGF-β1）蛋白表达水平。
与对照组相比，合剂组心肌组织损伤较轻，心肌亚细胞结构损伤程度较轻；胶原纤维含量和细胞凋亡减少；TSP-1mRNA 和 TRB-3mRNA 表达水平、心肌 TSP-1、TGF-β1、TRB-3 和糜酶表达水平以及心肌 TSP-1、A-TGFβ1 和 L-TGF-β1 蛋白的平均表达水平均降低。
生脉散合丹参饮可通过多种途径抑制 DCM 大鼠心肌纤维化过程，显著延缓高血糖大鼠 DCM 的发生和进展。
